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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4°C, protect from light
- 英文名：
2,2-Diphenyl-1-picrylhydrazyl
- 库存：
货期：1-2天
- 供应商：
MedChemExpress LLC
- CAS号：
1898-66-4
- 规格：
10 mM * 1 mL/5 mg/50 mg/100 mg
| 规格： | 10 mM * 1 mL | 产品价格： | ￥550.0 |
|---|---|---|---|
| 规格： | 5 mg | 产品价格： | ￥500.0 |
| 规格： | 50 mg | 产品价格： | ￥1000.0 |
| 规格： | 100 mg | 产品价格： | ￥1250.0 |
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DPPH
CAS No. : 1898-66-4
MCE 国际站：DPPH
产品活性：DPPH (2,2-Diphenyl-1-picrylhydrazyl) 是一种稳定的自由基，可用于测量抗氧化剂的自由基清除活性。DPPH 中氮原子的奇数电子通过从抗氧化剂吸收氢原子而还原为相应的肼。DPPH 方法可用于水性和非极性有机溶剂中，并可用于检查亲水性和亲脂性抗氧化剂。
研究领域：Others
作用靶点：Others
In Vitro: DPPH shows a strong absorption band at 517 nm due to its odd electron and solution appears a deep violet colour, the absorption vanishes as the electron pairs off. The resulting decolorization is stoichiometric with respect to the number of electrons taken up. The alcoholic solutions of 0.5 mM are densely colored and at this concentration, the Lambert-Beer law is obeyed over the useful range of absorption.
DPPH assay is a rapid, simple, inexpensive and widely used method to measure the ability of compounds to act as free radical scavengers or hydrogen donors, and to evaluate antioxidant activity of foods. It can also be used to quantify antioxidants in complex biological systems, for solid or liquid samples. This method is easy and applies to measure the overall antioxidant capacity and the free radical scavenging activity of fruit and vegetable juice. This assay has been successfully utilized for investigating antioxidant properties of wheat grain and bran, vegetables, conjugated linoleic acids, herbs, edible seed oils, and flours in several different solvent systems including ethanol, aqueous acetone, methanol, aqueous alcohol and benzene. It is a convenient method for the antioxidant assay of cysteine, glutathione, ascorbic acid, tocopherol and polyhydroxy aromatic compounds, for olive oil, fruits, juices and wines.
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文献和实验and microlocalization of antioxidants. We also present here a comparison of OxHLIA with other common methods (DPPH, ABTS•+ , and ORAC assays).
Separation of Phenylpropanoids and Evaluation of Their Antioxidant Activity
the highly oxygenated phenylpropanoids glycosides from the butanolic and water extracts. The antioxidant activity of the different phenylpropanoids is also reported on the basis of DPPH, superoxide anion scavenging and Fe2+ -chelating assays
the antioxidant potential of polyphenols are based on in vitro assay procedures (TEAC, ORAC, FRAP, DPPH). However, the antioxidant potential assessed by these methods does not correlate with the biological activity observed in vivo. Eukaryotic cells display
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