细胞名称: ATCC CRL-1458(L6)细胞 种属来源: 大鼠
组织来源: 骨骼肌
细胞形态: 成肌细胞
生长特性: 贴壁生长
培 养 基: DMEM培养基,90%;FBS,10%。
生长条件: 气相:空气,95%;二氧化碳,5%; 温度:37 ℃,
传代方法: 1:2至1:6,每周2次。
冻存条件: 90% 完全培养基+10% DMSO,液氮储存
支原体检测: 阴性
安全等级: 1
应 用: 该细胞可以作为转染宿主细胞。
备 注:
Nucleotide (GenBank) : M87067 R.norvegicus activin type IIB receptor mRNA.
Nucleotide (GenBank) : X57986 Rat mRNA for C alpha subunit of the cAMP-dependent protein kinase.
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Yaffe D. Retention of differentiation potentialities during prolonged cultivation of myogenic cells. Proc. Natl. Acad. Sci. USA 61: 477-483, 1968. PubMed: 5245982
Osawa H, et al. Identification and characterization of basal and cyclic AMP response elements in the promoter of the rat hexokinase II gene. J. Biol. Chem. 271: 17296-17303, 1996. PubMed: 8663388
Osawa H, et al. Analysis of the signaling pathway involved in the regulation of hexokinase II gene transcription by insulin. J. Biol. Chem. 271: 16690-16694, 1996. PubMed: 8663315