细胞名称: ATCC CRL-1378(RBL-1)大鼠嗜碱性粒细胞性白血病细胞 种属来源: 大鼠
组织来源: 嗜碱性粒细胞
疾病特征: 嗜碱性粒细胞性白血病
细胞形态: 淋巴母细胞样
生长特性: 悬浮生长
培 养 基: MEM培养基,90%;FBS,10%。
生长条件: 气相:空气,95%;二氧化碳,5%; 温度:37 ℃,
传代方法: 1:2至1:6,每周2次。
冻存条件: 90% 完全培养基+10% DMSO,液氮储存
支原体检测: 阴性
安全等级: 1
应 用: 该细胞可以作为转染宿主细胞。
备注:
Nucleotide (GenBank) : NM_031580 Rattus norvegicus glucose regulated protein, 58 kDa (Grp58), mRNA.
Nucleotide (GenBank) : X12355 Rat mRNA for phosphoinositide-specific phospholipase C form-I (PI-PLC I).
参考文献: Kulczycki A Jr., et al. The interaction of IgE with rat basophilic leukemia cells. I. Evidence for specific binding of IgE. J. Exp. Med. 139: 600-616, 1974. PubMed: 4812630
Barsumian EL, et al. IgE-induced histamine release from rat basophilic leukemia cell lines: isolation of releasing and nonreleasing clones. Eur. J. Immunol. 11: 317-323, 1981. PubMed: 6166481
Eccleston E, et al. Basophilic leukaemia in the albino rat and a demonstration of the basopoietin. Nat. New Biol. 244: 73-76, 1973.