细胞名称: ATCC CCL-2.2(HeLa S3)细胞 种属来源: 人
组织来源: 子宫
疾病特征: 宫颈癌
细胞形态: 上皮细胞样
生长特性: 贴壁生长
培 养 基: F-12K,90%;FBS,10%。
生长条件: 气相:空气,95%;二氧化碳,5%; 温度:37 ℃,
传代方法: 1:2至1:6,每周2次。
冻存条件: 90% 完全培养基+10% DMSO,液氮储存
支原体检测: 阴性
安全等级: 2
应 用: 该细胞可以作为转染宿主细胞。
STR:
Amelogenin: X
CSF1PO: 9,10
D13S317: 13.3
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
同工酶:
G6PD, A
备 注:
Nucleotide (GenBank) : Y09723 H.sapiens mRNA for Miz-1 protein.
Nucleotide (GenBank) : Y08698 H.sapiens mRNA for RanBP3 (59 kDa).
Nucleotide (GenBank) : Y08699 H.sapiens mRNA for RanBP3, splice variant.
Nucleotide (GenBank) : U25182 Human antioxidant enzyme AOE37-2 mRNA, complete cds.
Nucleotide (GenBank) : U33822 Human tax1-binding protein TXBP181 mRNA, complete cds.
Nucleotide (GenBank) : U43430 Human epsilon isoform 14-3-3 protein mRNA, complete cds.
Nucleotide (GenBank) : Y08697 H.sapiens mRNA for RanBP3 small splice variant (53 kDa).
Nucleotide (GenBank) : L15328 Saccharomyces cerevisiae RNA helicase gene, complete cds.
Nucleotide (GenBank) : U33821 Homo sapiens tax1-binding protein TXBP151 mRNA, complete cds.
Nucleotide (GenBank) : NM_003443 Homo sapiens zinc finger protein 151 (pHZ-67) (ZNF151), mRNA.
Nucleotide (GenBank) : AF034102 Homo sapiens NBMPR-insensitive nucleoside transporter ei (ENT2) mRNA, complete cds.
Nucleotide (GenBank) : NM_006024 Homo sapiens Tax1 (human T-cell leukemia virus type I) binding protein 1 (TAX1BP1), mRNA.
参考文献: Chen TR. Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes. Cytogenet. Cell Genet. 48: 19-24, 1988. PubMed: 3180844
Boshart M, et al. A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO J. 3: 1151-1157, 1984. PubMed: 6329740
Puck TT, et al. Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer. J. Exp. Med. 103: 273-283, 1956. PubMed: 13286432
Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217
Puck TT, Marcus PI. A rapid method for viable cell titration and clone production with HeLa cells in tissue culture: the use of x-irradiated cells to supply conditioning factors. Proc. Natl. Acad. Sci. USA 41: 432-437, 1955.
特点和简介 ATCC CCL-2.2(HeLa S3)细胞该细胞是1955年由Puck TT, Marcus PI和Cieciura SJ建系的,含HPV-18序列;角蛋白阳性;可用于与染色体突变、细胞营养、集落形成相关的哺乳动物细胞的克隆分析。