Exosome Isolation kit (for c

RNA Isolation From Animal tissue or cell culture

mL collection tube. The maximum capacity of the spin cartridge is 750 ul. A precipitate may form on addition of ethanol in step 5. Vortex and add the entire mixture to the column. With the spin column inside a 2ml collection tube (supplied with kit

Cell  Isolation  Techniques

for recirculating the buffers, media and enzymes. Please refer to referenced texts for additional information and guidance. Equilibration with 95%O2 :5%CO2 In many cell isolation procedures it is important to the survival of the tissue

CellTrace CFSE Cell Proliferation Kit

Materials Needed but Not Included: Cells of interest as a single-cell suspension PBS/0.1% BSA 5 mM stock solution of CFSE probe (see Reagent Preparation) Culture media for cells of interest Flow cytometer with 488