DYKDDDDK Tag Antibody (Binds t
o same epitope as Sigma's Anti-FLAG M2 Antibody) (Alexa Fluor 647 Conjugate)- 询价
- Cell Signaling Technology
- 3916
- USA
- 2025年10月01日
- 免疫荧光（IF）,流式细胞（Flow Cyt）
- 不限
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- 详细信息
- 技术资料
- 抗体英文名：
DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG M2 Antibody) (Alexa Fluor 647 Conjugate)
- 抗原：
/
- 应用范围：
免疫荧光（IF）,流式细胞（Flow Cyt）
- 供应商：
CST
- 适应物种：
不限
- 库存：
大量
- 抗原来源：
/
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 是否单克隆：
0
- 保存条件：
-20°c
- 规格：
100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Complementary Reagents
DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) (Alexa Fluor® 647 Conjugate) #3916
| Applications | Reactivity | Sensitivity | Source |
|---|---|---|---|
| IF-IC F | All | Transfected Only | Rabbit |
Applications Key: IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
Protocols
- 3916:
- Flow , Immunofluorescence
Specificity / Sensitivity
DYKDDDDK Tag Antibody (Alexa Fluor® 647 Conjugate) detects exogenously expressed DYKDDDDK proteins in cells. The antibody recognizes the DYKDDDDK peptide (the same epitope recognized by Sigma's Anti-FLAG® antibodies) fused to either the amino- or carboxy-terminus of targeted proteins. The binding specificity of this antibody is NOT dependent on the presence of divalent metal cations.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic DYKDDDDK peptide. Antibodies are purified by protein A and peptide affinity chromatography.
Flow Cytometry
Flow cytometric analysis of COS cells, either untransfected (blue) or transfected with a DYKDDDDK-tagged fusion protein, using DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) (Alexa Fluor® 647 Conjugate) (green).
IF-IC
Confocal immunofluorescent analysis of COS cells transiently transfected with a FLAG-tagged protein using DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) (Alexa Fluor® 647 Conjugate) (blue pseudocolor). Red = Propidium Iodide (fluorescent DNA dye).
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in cells transiently transfected with a DYKDDDDK-tagged fusion protein.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (1).
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 2368 DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody)
- 3768 DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) (Alexa Fluor® 555 Conjugate)
- 2908 DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) (Biotinylated)
- 2044 DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) (HRP Conjugate)
Anti-FLAG® is a registered trademark of Sigma-Aldrich.
For Research Use Only. Not For Use In Diagnostic Procedures.
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