PTMScan Phospho-ATM/ATR Subs

Product Pathways - Motif Antibodies

• Antibodies for PTMScan^®
• more info
• application references

PTMScan^® Phospho-ATM/ATR Substrate (S*Q) Immunoaffinity Beads #9884

Services Information

This product is not for individual sale. It is only available as a component of the PTMScan^® Proteomics System . PTMScan^® Proteomics System orders must be priced out individually. Please email us at ptmscan@cellsignal.com to receive the most accurate pricing.

Description

PTMScan^® Immunoaffinity Beads are custom preparations of motif antibodies coupled to protein A beads. They are intended only for use for PTMScan^® and are available as components of the PTMScan^® Proteomics System.

Specificity / Sensitivity

PTMScan^® Phospho-ATM/ATR Substrate Motif (pS/pTQ) Immunoaffinity Beads detect and capture endogenous levels of peptide derived from protease digested cellular proteins containing phospho-Ser/Thr followed by Gln at the +1 position. The antibody does not cross-react with corresponding nonphosphorylated sequences or with other phospho-Ser/Thr-containing motifs. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Background

Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair (1). The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1, and Chk1 (1,2) The essential requirement for the substrates of ATM/ATR is S*/T*Q. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate recognition by these kinases. Positively charged residues surrounding the S*/T*Q are negative determinants for substrate phosphorylation (3). The complex phenotype of AT cells suggests that it likely has additional substrates (3). To better understand the kinase and identify substrates for ATM and the related kinase ATR, CST has developed antibodies that recognize phosphorylated serine or threonine in the S*/T*Q motif.

1. Kastan, M.B. and Lim, D.S. (2000) Nature Rev. Mol. Cell Biol. 1, 179-186.
2. Zhao, H. and Piwnica-Worms, H. (2001) Mol. Cell. Biol. 21, 4129-4139.
3. Kim, S. T. et al. (1999) J. Biol. Chem. 274, 37538-37543.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

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For Research Use Only. Not For Use In Diagnostic Procedures.