Phospho-Ezrin (Thr567)/Radixin
(Thr564)/Moesin (Thr558) (48G2) Rabbit mAb (ELISA Specific)- 询价
- Cell Signaling Technology
- 3726
- USA
- 2025年12月22日
- E-P
- H,M,R,B
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (48G2) Rabbit mAb (ELISA Specific)
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Thr567 of human ezrin protein
- 应用范围：
E-P
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 库存：
大量
- 供应商：
CST
- 适应物种：
H,M,R,B
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: E-P=ELISA (Peptide)
Reactivity Key: H=Human M=Mouse R=Rat B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| E-P | H (M) (R) (B) | Endogenous | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (41A3) Rabbit mAb (ELISA Specific) is phospho-specific by Peptide-ELISA and exhibits a large signal to noise window. The antibody does not recognize the non-phosphorylated peptide in peptide based ELISA (see figure below). |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr567 of human ezrin protein. ELISA-Peptide
Validation of Phospho-Ezrin (Thr567)/ Radixin (Thr564)/Moesin (Thr558) (48G2) Rabbit mAb (ELISA Specific) in peptide DELFIA® assay using phospho- and nonphospho-peptide controls, and DELFIA® secondary antibodies (available from Perkin Elmer Life and Analytical Sciences). |
| Background | The ezrin, radixin, and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling, and microvilli formation (1). ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts the amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6).
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| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
the phosphorylation site; therefore, substrate?directed, phosphorylation?state?sensitive, motif?specific (?phospho?motif?) antibodies represent powerful tools to identify novel kinase substrates and to investigate mechanisms of substrate phosphorylation
(Ser473)(193H12)Rabbit mAb，1:300,稀释，二抗是驴抗兔，1：2000，我是实验室的新手，刚呆俩月，受 此挫折，一头雾水，请过来人或者有经验的同学帮忙解答怎样解决，非常感谢！ 碧峤 pAkt有两个位点，Ser473和Thr308，其中Ser473相对容易显色。如果显色失败，可能的原因:1 上样量过少，我们一般都是30μg；2 一抗孵育时间过短，我们一般24h~48h；3 一抗是否工作有待排除 米宝
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 and Total-p38 antibodies (C). Cell-Based Elisa将小鼠巨噬细胞4/4细胞种在96孔板内，每个孔种5 x 104 cells/cm2细胞。细胞用不同浓度的anisomycin刺激，我们来监测P38 MAPK和JNK两种蛋白的变化。按照试剂盒的实验步骤，分别固定，打孔，猝灭，封闭，然后一抗孵育，二抗孵育，最后显色，用常规的酶标仪读取数值。最后数据再和试剂盒提供的结晶紫核染料结果做纠正，最后得出以上数据。Quantitative
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