SET8 (C18B7) Rabbit mAb

2996:

Immunofluorescence , Western Blotting

SET8 (C18B7) Rabbit mAb detects endogenous levels of total SET8 protein (both isoforms).

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to human SET8 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from HCT116 and RAW cells using SET8 (C18B7) Rabbit mAb.

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with 0.5% Triton X-100 for 10 minutes prior to fixation according to Tardat et al. (2007) J. Cell Biol. 179, 1413-26 (right), using SET8 (C18B7) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

SET domain-containing lysine methyltransferase 8 (SET8), also known as PR/SET domain-containing protein 7 (PR/SET7), is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Drosophila Su[var]3-9, Enhancer of zeste, and Trithorax proteins (1-3). SET8 is a single-subunit enzyme that mono-methylates histone H4 on Lys20, preferably on nucleosomal substrates (1-3). SET8 protein levels and Histone H4 Lys20 methylation are cell cycle regulated, both increasing in S phase and peaking at G2/M phase (4,5). SET8 interacts with the PCNA protein, associates with sites of active DNA synthesis and is required for DNA replication and genome stability during S phase (5-7). Inhibition of SET8 using shRNA results in arrest of replication forks, induction of double-stranded DNA breaks and a Chk1-mediated cell-cycle arrest in S and G2/M phases of the cell cycle (6,7). Furthermore, SET8 methylates p53 on Lys382, down regulating the pro-apoptotic and checkpoint activation functions of p53 (8). In response to DNA damage, SET8 expression levels decrease, allowing p53 to activate checkpoints and/or apoptosis (8). Both the methylation of histone H4 Lys20 and p53 appear to be important for the functions of SET8 in S phase.

1. Fang, J. et al. (2002) Curr Biol 12, 1086-99.
2. Xiao, B. et al. (2005) Genes Dev 19, 1444-54.
3. Couture, J.F. et al. (2005) Genes Dev 19, 1455-65.
4. Rice, J.C. et al. (2002) Genes Dev 16, 2225-30.
5. Huen, M.S. et al. (2008) J Biol Chem 283, 11073-7.
6. Tardat, M. et al. (2007) J Cell Biol 179, 1413-26.
7. Jørgensen, S. et al. (2007) J Cell Biol 179, 1337-45.
8. Shi, X. et al. (2007) Mol Cell 27, 636-46.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

• 2991 SUV39H1 Histone Methyltransferase Antibody
• 3306 G9a/EHMT2 (C6H3) Rabbit mAb
• 3147 Ezh2 (AC22) Mouse mAb
• 2813 SET7/SET9 Antibody
• 2196 ESET (C1C12) Rabbit mAb
• 2586 PCNA (PC10) Mouse mAb
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.