UBE2N (D2A1) Rabbit mAb

6999:

Immunoprecipitation , Western Blotting

UBE2N (D2A1) Rabbit mAb recognizes endogenous levels of total UBE2N protein. This antibody does not cross-react with UBE2D family members.

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human UBE2N protein.

Western Blotting

Western blot analysis of extracts from 293T cells, either mock transfected or transfected with a Myc/DDK-tagged cDNA expression construct encoding full-length human UBE2N, UBE2D1, UBE2D2, UBE2D3, or UBE2D4, using UBE2N (D2A1) Rabbit mAb (upper) and DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG^® M2 Antibody) #2368 (lower).

Western Blotting

Western blot analysis of extracts from various cell lines using UBE2N (D2A1) Rabbit mAb.

Protein ubiquitination is an important posttranslational modification that regulates protein function and fate (1). Ubiquitin (Ub) can be conjugated to target proteins in either monomeric or polymeric forms. There are several different lysine residues within Ub that can be used as conjugation sites for poly-Ub chain formation. Different poly-Ub linkages mediate different functions of the target protein ranging from alterations in protein function to degradation (2). UBE2N/Ubc13 is a ubiquitin-E2-conjugating enzyme that catalyzes K63-linked poly-Ub chain formation (1,2). UBE2N forms a heterodimer with MMS2 or Uev1A to exert its E2 ligase function. The UBE2N/MMS2 and UBE2N/Uev1A heterodimers catalyze different modes of target protein ubiquitination to mediate various signaling pathways (3-5) including: DNA damage and recombination, p53 and check point control, the cell cycle (6-10), immunoreceptor signaling (11,12), and endocytosis (13). Most recently, UBE2N was shown to play an important role in inflammatory signaling by promoting K63-linked ubiquitination and activation of IKK downstream of the IL-1β receptor (14). Furthermore, interaction of UBE2N with the Triad1 E3 protein-ubiquitin ligase was shown to play an important role in myelopoiesis (15).

1. Herrmann, J. et al. (2007) Circ Res 100, 1276-91.
2. Wilkinson, K.D. et al. (2005) EMBO Rep 6, 815-20.
3. Hofmann, R.M. and Pickart, C.M. (1999) Cell 96, 645-53.
4. Deng, L. et al. (2000) Cell 103, 351-61.
5. Andersen, P.L. et al. (2005) J Cell Biol 170, 745-55.
6. Zhao, G.Y. et al. (2007) Mol Cell 25, 663-75.
7. Kolas, N.K. et al. (2007) Science 318, 1637-40.
8. Laine, A. et al. (2006) Mol Cell Biol 26, 8901-13.
9. Huen, M.S. et al. (2008) Mol Cell Biol 28, 6104-12.
10. Loring, G.L. et al. (2008) Cell Cycle 7, 96-105.
11. Yamamoto, M. et al. (2006) Nat Immunol 7, 962-70.
12. Yamamoto, M. et al. (2006) J Immunol 177, 7520-4.
13. Duncan, L.M. et al. (2006) EMBO J 25, 1635-45.
14. Xu, M. et al. (2009) Mol Cell 36, 302-14.
15. Marteijn, J.A. et al. (2009) Leukemia 23, 1480-9.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

• 4919 Ubc13 Antibody
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.