- 询价
- Cell Signaling Technology
- 2551
- USA
- 2025年12月12日
- W, IF-IC
- Rabbit
- H,M,R,Mk,X
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- 详细信息
- 技术资料
- 抗体英文名：
eEF1A Antibody
- 抗原：
synthetic peptide corresponding to a sequence at the C-terminal end of human eEF1A
- 应用范围：
W, IF-IC
- 宿主：
Rabbit
- 库存：
大量
- 适应物种：
H,M,R,Mk,X
- 级别：
详见MSDS文件
- 供应商：
CST
- 保质期：
详见说明书
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey X=Xenopus
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC | H M R Mk (X) | Endogenous | 50 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | eEF1A Antibody detects endogenous levels of total eEF1A protein. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence at the C-terminal end of human eEF1A. Antibodies are purified by peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from various cell types using eEF1A Antibody. IF-IC
Confocal immunofluorescent analysis of HeLa cells using eEF1A Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | Translation is the process where amino acid residues are assembled into polypeptides on ribosomes. This process is generally divided into three stages: initiation, elongation and termination. During elongation, mRNA and tRNA pair at the two active sites (A and P sites) on the ribosome. A number of eukaryotic elongation factors (eEFs) are involved in this process in mammalian cells (1). eEF1A, also called elongation factor Tu (EF-Tu), binds GTP and interacts with amino acyl-tRNAs to promote recruitment of amino acyl-tRNAs to the A-site of the ribosome (1). After GTP hydrolysis, GDP-eEF1A leaves the ribosome and is later converted back to the GTP-eEF1A by eEF1B (1). Studies have shown that eEF1A is phosphorylated under certain conditions, indicating that its activity is regulated at the post-translational level (2,3). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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