- 询价
- Cell Signaling Technology
- 4890
- USA
- 2025年11月19日
- W, IP, IHC-P, IF-IC, F
- Rabbit
- H,M,R
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
UBE1a Antibody
- 抗原：
synthetic peptide corresponding to the amino terminus of human UBE1
- 应用范围：
W, IP, IHC-P, IF-IC, F
- 宿主：
Rabbit
- 供应商：
CST
- 级别：
详见MSDS文件
- 适应物种：
H,M,R
- 库存：
大量
- 保质期：
详见说明书
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IHC-P IF-IC F | H M R | Endogenous | 117 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | UBE1a Antibody detects endogenous levels of total UBE1a protein. This antibody does not cross-react with UBE1b. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human UBE1. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from K562 and SK-N-MC cells using UBE1a Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded glioblastoma using UBE1a Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using UBE1a Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse brain using UBE1a Antibody in the presence of control peptide (left) or antigen specific peptide (right). Flow Cytometry
Flow cytometric analysis of K562 cells using UBE1a Antibody (blue) compared to a nonspecific negative control antibody (red). IF-IC
Confocal immunofluorescent analysis of SK-N-MC cells using UBE1a Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). |
| Background | Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the ubiquitin-activating enzyme (UBE1 or E1). The activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the ε-amino group of the target protein lysine residue (1-3). Combinatorial interactions of different E2 and E3 proteins result in substrate specificity (4). UBE1 has two isofoms: UBE1a is a nuclear protein of 117 kDa while UBE1b is a nuclear and cytoplasmic protein of 110 kDa (5). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease
技术资料暂无技术资料 索取技术资料
