- 询价
- Cell Signaling Technology
- 4870
- USA
- 2025年11月17日
- W, IF-IC, F
- Rabbit
- H,M,R,Mk,Dm
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- 详细信息
- 技术资料
- 抗体英文名：
HSP60 (D307) Antibody
- 抗原：
synthetic peptide surrounding Asp307 of human HSP60
- 应用范围：
W, IF-IC, F
- 宿主：
Rabbit
- 库存：
大量
- 供应商：
CST
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 适应物种：
H,M,R,Mk,Dm
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Dm=D. melanogaster
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC F | H M R Mk Dm | Endogenous | 60 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | HSP60 (D307) Antibody detects endogenous levels of total HSP60 protein. This antibody does not cross-react with other HSPs. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Asp307 of human HSP60. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from various cell types using HSP60 (D307) Antibody. Flow Cytometry
Flow cytometric analysis of HeLa cells using HSP60 (D307) Antibody (blue) compared to a nonspecific negative control antibody (red). IF-IC
Confocal immunofluorescent analysis of HeLa cells using HSP60 (D307) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | In both prokaryotic and eukaryotic cells the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones (1-3). HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria (4). It is now clear that a significant amount of HSP60 is also present in the cytosol of many cells and that it is induced by stress, inflammatory and immune responses, autoantibodies correlated with Alzheimer's, coronary artery diseases, MS, and diabetes (5-8).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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