Applications Key: W=Western Blotting IP=Immunoprecipitation Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
hnRNP Q/R (D18B2) Rabbit mAb recognizes endogenous levels of total hnRNP Q and hnRNP R proteins.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys371 of human hnRNP Q protein.
Western Blotting
Western blot analysis of extracts from SW620 and HCT 116 cells using hnRNP Q/R (D18B2) Rabbit mAb.
Background
Heterogeneous nuclear ribonucleoprotein Q and R belong to a family of hnRNP proteins that are involved in RNA binding, RNA biosynthesis, and mRNA transport from the nucleus to the cytoplasm (1-3). These two proteins are encoded by different genes but have 83% homology. hnRNP Q has three alternative splice variants (hnRNP Q1-3) (1-3). Methylation of carboxy-terminal arginine residues is required for nuclear localization (4). hnRNP Q binds to AU-rich mRNA in conjunction with AUF1 and regulates mRNA decay (5). hnRNP Q isoforms play a crucial role in mediating nuclear function of survival of motor neuron (SMN) complex (6,7) and modulating RNA biosynthesis and hepatitis C virus replication (8). hnRNP R was identified recently and its function is still under investigation (9), however hnRNP R does not duplicate the biological function of hnRNP Q. Both hnRNP Q and R are present in cytoplasmic mRNP granules containing untranslated mRNAs (10) and both interact with SMN (11).