NLRX1 Antibody

8583:

Immunoprecipitation , Western Blotting

NLRX1 Antibody recognizes endogenous levels of total NLRX1 protein. This antibody cross-reacts with a 65 kDa protein of unknown origin.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys441 of human NLRX1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from various cell lines using NLRX1 Antibody.

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a cDNA construct expressing full-length human NLRX1 (hNLRX1; +), using NLRX1 Antibody.

The nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) family of proteins is a diverse family of cytoplasmic innate immune receptors. They are characterized by the presence of an amino-terminal effector domain, which is often either a caspase activation and recruitment domain (CARD) or a pyrin domain (PYD), followed by a NOD and carboxy-terminal leucine-rich-repeat (LRR) domain involved in recognition of pathogen-associated molecular patterns (PAMPs) (1). NLR proteins play a variety of roles during the innate immune response including pathogen sensing, transcriptional activation of proinflammatory cytokines through NF-κB, transcriptional activation of type I interferons through IRFs, and formation of inflammasomes leading to activation of inflammatory caspases (1-7).

NLRX1 (CLR11.3/NOD26/NOD5/NOD9) is unique among NLR family members in that it contains an amino-terminal mitochondrial targeting sequence resulting in localization to the mitochondria (8,9). In contrast to most NLR proteins, NLRX1 has been shown to act as a negative regulator of innate immune responses through inhibition of MAVS-Rig-I signaling, as well as inhibition of Toll-like receptor (TLR)-mediated NF-κB activation (9-11). In addition, overexpression of NLRX1 enhanced the production of reactive oxygen species (ROS), resulting in prolonged NF-κB and JNK signaling in response to TNF-α (8).

1. Elinav, E. et al. (2011) Immunity 34, 665-79.
2. Inohara, N. et al. (1999) J Biol Chem 274, 14560-7.
3. Ogura, Y. et al. (2001) J Biol Chem 276, 4812-8.
4. Sabbah, A. et al. (2009) Nat Immunol 10, 1073-80.
5. Mariathasan, S. et al. (2004) Nature 430, 213-8.
6. Agostini, L. et al. (2004) Immunity 20, 319-25.
7. Martinon, F. et al. (2002) Mol Cell 10, 417-26.
8. Tattoli, I. et al. (2008) EMBO Rep 9, 293-300.
9. Moore, C.B. et al. (2008) Nature 451, 573-7.
10. Allen, I.C. et al. (2011) Immunity 34, 854-65.
11. Xia, X. et al. (2011) Immunity 34, 843-53.

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• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.