STIM1 (D88E10) Rabbit mAb

5668:

Immunofluorescence , Immunoprecipitation , Western Blotting

STIM1 (D88E10) Rabbit mAb recognizes endogenous levels of total STIM1 protein. The antibody does not cross-react with STIM2.

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro622 of human STIM1 protein.

Western Blotting

Western blot analsyis of extracts from various cell lines using STIM1 (D88E10) Rabbit mAb.

IF-IC

Confocal immunofluorescent analysis of COS-7 cells, untreated (left) or treated with histamine and thapsigargin (right) using STIM1 (D88E10) Rabbit mAb (green). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Ca²⁺ is a key second messenger in many intracellular signaling pathways. Ca²⁺ signals control many cellular functions ranging from short-term responses such as contraction and secretion to longer-term regulation of cell growth and proliferation (1,2). Stromal interaction molecules (STIMs) function as Ca²⁺ sensors that detect changes in Ca²⁺ content in intracellular Ca²⁺ stores (3). STIM1 is conserved, ubiquitously expressed, and functions as an endoplasmic reticulum (ER) Ca²⁺ sensor that migrates from the ER Ca²⁺ store to the plasma membrane where it activates calcium-release-activated calcium (CRAC) channels when the ER Ca²⁺ store is low (4). STIM1 is a potential tumor suppressor; defects in STIM1 may cause rhabdomyosarcoma and rhabdoid tumors (5). STIM1 can either homodimerize or form heterodimers with STIM2. STIM2 possesses a high sequence identity to STIM1 and can function as an inhibitor of STIM1-mediated plasma membrane store-operated Ca²⁺ entry (6). However, further investigation is required to elucidate the true physiological function of STIM2.

1. Berridge, M.J. et al. (2000) Nat Rev Mol Cell Biol 1, 11-21.
2. Berridge, M.J. et al. (2003) Nat Rev Mol Cell Biol 4, 517-29.
3. Zheng, L. et al. (2008) Biochem Biophys Res Commun 369, 240-6.
4. Zhang, S.L. et al. (2005) Nature 437, 902-5.
5. Manji, S.S. et al. (2000) Biochim Biophys Acta 1481, 147-55.
6. Soboloff, J. et al. (2006) Curr Biol 16, 1465-70.

• Mungai, P.T. et al. (2011) Mol Cell Biol 31, 3531-45. Applications: IF-IC (In Cells) Western Blotting

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For Research Use Only. Not For Use In Diagnostic Procedures.