hnRNP E1 Antibody

8534:

Immunoprecipitation , Western Blotting

hnRNP E1 Antibody recognizes endogenous levels of total hnRNP E1 protein.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys237 of human hnRNP E1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from various cell lines using hnRNP E1 Antibody.

hnRNP E1 is a member of the hnRNP family of proteins that are involved in pre-mRNA processing and mRNA export, localization, stability, and translation (1-6). hnRNP E1 exerts a wide range of biological functions, such as transcriptional activation of mouse MOR gene expression (7), attenuation of alternative splicing of GHR pseudoexon expression (8), stabilization of collagen I and II (9), beta-globin (10), and androgen receptor (11) mRNAs, and regulation of translation of various genes including Dab2, ILEI, and Bag-1 (12,13). hnRNP E1 is ubiquitously expressed. Phosphorylation of hnRNP E1 affects its RNA binding affinity (13,14).

1. Cáceres, J.F. et al. (1994) Science 265, 1706-9.
2. Gallouzi, I.E. and Steitz, J.A. (2001) Science 294, 1895-901.
3. Carson, J.H. et al. (2001) Results Probl Cell Differ 34, 69-81.
4. Xu, K. et al. (2001) J Immunol 166, 6358-66.
5. Habelhah, H. et al. (2001) Nat Cell Biol 3, 325-30.
6. Dreyfuss, G. et al. (2002) Nat Rev Mol Cell Biol 3, 195-205.
7. Kim, S.S. et al. (2005) Mol Pharmacol 68, 729-36.
8. Akker, S.A. et al. (2007) Mol Endocrinol 21, 2529-40.
9. Thiele, B.J. et al. (2004) Circ Res 95, 1058-66.
10. Yu, J. and Russell, J.E. (2001) Mol Cell Biol 21, 5879-88.
11. Yeap, B.B. et al. (2002) J Biol Chem 277, 27183-92.
12. Pickering, B.M. et al. (2003) Nucleic Acids Res 31, 639-46.
13. Chaudhury, A. et al. (2010) Nat Cell Biol 12, 286-93.
14. Leffers, H. et al. (1995) Eur J Biochem 230, 447-53.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 9304 hnRNP A2/B1 (2A2) Mouse mAb
• 8443 hnRNP A1 (D21H11) Rabbit mAb
• 4296 hnRNP A1 (K350) Antibody
• 5380 hnRNP A1 (R196) Antibody
• 8588 hnRNP Q/R (D18B2) Rabbit mAb
• 5351 hnRNP Q/R Antibody
• 5545 hnRNP A0 (D8A3) XP^® Rabbit mAb
• 5600 hnRNP A0 (D26A2) Rabbit mAb
• 4046 hnRNP A0 Antibody
• 4783 hnRNP LL Antibody
• 4699 hnRNP K (A222) Antibody
• 4675 hnRNP K (R332) Antibody

For Research Use Only. Not For Use In Diagnostic Procedures.