- 询价
- Cell Signaling Technology
- 9567
- USA
- 2025年11月04日
- W, IP
- Rabbit
- H,R,M,C,X,Z
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- 详细信息
- 技术资料
- 抗体英文名：
Phospho-β-Catenin (Ser675) Antibody
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Ser675 of human β-catenin
- 应用范围：
W, IP
- 宿主：
Rabbit
- 适应物种：
H,R,M,C,X,Z
- 保质期：
详见说明书
- 库存：
大量
- 级别：
详见MSDS文件
- 供应商：
CST
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat C=Chicken X=Xenopus Z=Zebrafish
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H R (M) (C) (X) (Z) | Endogenous | 92 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-β-Catenin (Ser675) Antibody detects endogenous levels of β-catenin only when phosphorylated at Ser675. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser675 of human β-catenin. Antibodies are purified by peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from SK-N-MC cells, treated with forskolin (FSK) for 30 minutes or λ phosphatase for 1 hour, using Phospho-β-Catenin (Ser675) Antibody (upper) or β-Catenin Antibody (Amino-terminal Antigen) #9581 (lower). Western Blotting
COS-7 cells were transfected with cDNA encoding DYKDDDDK-tagged wild type (WT) β-catenin or Ser-to-Ala β-catenin mutants as indicated. Cells were stimulated with 10 mM forskolin (FSK) for 5 minutes and lysed. β-catenin or its mutants were immunoprecipitated with DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #2368 and analyzed by western blotting using Phospho-β-Catenin (Ser552) Antibody #9566, Phospho-β-Catenin (Ser675) Antibody or DYKDDDDK Tag Antibody as indicated (Figures provided by Drs. Sebastien Taurin and Nickolai Dulin, Department of Medicine/Pulmonary, University of Chicago). |
| Background | β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3 (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8). PKA was shown to phosphorylate β-catenin at Ser675. Phosphorylation at Ser675 induces β-catenin accumulation in the nucleus and increases its transcriptional activity (9,10).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Anti-FLAG® is a registered trademark of Sigma-Aldrich. For Research Use Only. Not For Use In Diagnostic Procedures. |
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