- 询价
- Cell Signaling Technology
- 8418
- USA
- 2025年10月29日
- W, IP, IHC-P
- H,M,Mk
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
YAP/TAZ (D24E4) Rabbit mAb
- 抗原：
synthetic peptide corresponding to residues surrounding Asp362 of human TAZ protein
- 应用范围：
W, IP, IHC-P
- 级别：
详见MSDS文件
- 库存：
大量
- 供应商：
CST
- 适应物种：
H,M,Mk
- 保质期：
详见说明书
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human M=Mouse Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P | H M Mk | Endogenous | 50, 70 | Rabbit IgG |
| Protocols | |
|---|---|
| Specificity / Sensitivity | YAP/TAZ (D24E4) Rabbit mAb recognizes endogenous levels of total YAP and TAZ proteins. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp362 of human TAZ protein. Western Blotting
Western blot analysis of extracts from various cell lines using YAP/TAZ (D24E4) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using YAP/TAZ (D24E4) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lymphoma using YAP/TAZ (D24E4) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded COS-7 cell pellets, transfected with YAP (left) or TAZ (right), using YAP/TAZ (D24E4) Rabbit mAb. |
| Background | YAP (Yes-associated protein, YAP65) was identified based on its ability to associate with the SH3 domain of Yes. It also binds to other SH3 domain-containing proteins such as Nck, Crk, Src, and Abl (1). In addition to the SH3 binding motif, YAP contains a PDZ interaction motif, a coiled-coil domain, and WW domains (2-4). While initial studies of YAP all pointed towards a role in anchoring and targeting to specific subcellular compartments, subsequent studies showed that YAP is a transcriptional co-activator by virtue of its WW domain interacting with the PY motif (PPxY) of the transcription factor PEBP2 and other transcription factors (5,6). Upon phosphorylation at Ser127, YAP interacts with 14-3-3 in an Akt-dependent manner and suppresses p73-mediated apoptosis (6). YAP may also function as a transcriptional repressor (7) and is now recognized as a critical regulator of the Hippo (Mst1/Mst2) signaling pathway (8). YAP may therefore play an important role in both organ size determination and tumor suppression (8).TAZ is a closely related transcriptional co-activator also implicated in the Hippo signaling pathway (9). Taz contains a PDZ-binding motif, which shares homology with the WW domain of YAP, and has been proposed to modulate the switch between proliferation and differentiation of mesenchymal stem cells (MSCs) via interaction with the transcription factors Runx2 and PPARγ. This process is critical for normal tissue development and tumor suppression (10). Due to its role in the determination of MSC fate, TAZ may have clinical relevance to several human diseases caused by an imbalance of MSC differentiation (11).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
h）即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复：简单易行效果好，CST 推荐使用微波炉完成修复。合适的修复液：根据抗体说明书使用合适的修复液。用柠檬酸修复后，切片需浸泡在修复液中，自然冷却；而用 EDTA 修复后，切片可直接从修复缸中取出，直接进行下一步。注：使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb（#4267），EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
到 PVDF 膜上，同时减少蛋白不必要降解，这对于最终获得清晰、可信结果也是需要考虑因素。抗体信息：1.ACC1, Recombinant Rabbit monoclonal IgG. HuaAn. HuaAn biotechnology , inc.2.ATGL, Mouse mAb IgG1, Cat#:RT1058. HuaAn biotechnology , inc.3.p-PERK(Thr981), Rabbit Polyclonal IgG primary antibodies, Cat
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