- 询价
- Cell Signaling Technology
- 9408
- USA
- 2025年10月22日
- W
- H,M,R
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
NDRG1 (D6C2) Rabbit mAb
- 抗原：
synthetic peptide corresponding to residues surrounding Gly25 of human NDRG1 protein
- 应用范围：
W
- 库存：
大量
- 适应物种：
H,M,R
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 供应商：
CST
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R | Endogenous | 46, 48 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | NDRG1 (D6C2) Rabbit mAb recognizes endogenous levels of total NDRG1 protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly25 of human NDRG1 protein. Western Blotting
Western blot analysis of extracts from various cell lines and rat brain tissue using NDRG1 (D6C2) Rabbit mAb. |
| Background | N-myc downstream-regulated gene 1 (NDRG1), also termed Cap43, Drg1, RTP/rit42, and Proxy-1, is a member of the NDRG family, which is composed of four members (NDRG1-4) that function in growth, differentiation, and cell survival (1-5). NDRG1 is ubiquitously expressed and highly responsive to a variety of stress signals including DNA damage (4), hypoxia (5), and elevated levels of nickel and calcium (2). Expression of NDRG1 is elevated in N-myc defective mice and is negatively regulated by N- and c-myc (1,6). During DNA damage, NDRG1 is induced in a p53-dependent fashion and is necessary for p53-mediated apoptosis (4,7). Research studies have shown that NDRG1 may also play a role in cancer progression by promoting differentiation, inhibiting growth, and modulating metastasis and angiogenesis (3,4,6,8,9). Nonsense mutation of the NDRG1 gene has been shown to cause hereditary motor and sensory neuropathy-Lom (HMSNL), which is supported by studies demonstrating the role of NDRG1 in maintaining myelin sheaths and axonal survival (10,11). NDRG1 is up-regulated during mast cell maturation and its deletion leads to attenuated allergic responses (12). Both NDRG1 and NDRG2 are substrates of SGK1, although the precise physiological role of SGK1-mediated phosphorylation is not known (13). NDRG1 is phosphorylated by SGK1 at Thr328, Ser330, Thr346, Thr356, and Thr366. Phosphorylation by SGK1 primes NDRG1 for phosphorylation by GSK-3.
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验was an effective method for enriching phosphate-containing proteins. Further validating the method, this workflow was applied to probe changes in the activation patterns of intermediates involved in different signaling pathways, such as NDRG1 and stathmin, in liver
Methods for the Detection of D-Amino-Acid Oxidase
then twice for 5 min. It was incubated for 1 hr in TBS-T containing rabbit anti-hog D-amino-acid oxidase IgG (1/3,000 dilution) and quickly rinsed twice with TBS-T and further washed in TBS-T once for 15 min then twice for 5 min. The membrane was incubated
组织化学分析。（图 C）使用Anti-Erk1/2 Mouse mAb 鼠单抗进行目的蛋白检测；(图 D) 使用p44/42 MAPK （Erk1/2）Rabbit mAb 兔单抗进行目的蛋白检测。 图 7 免疫组化实验检测Akt表达 注：使用 Anti-Akt (pan) Rabbit mAb 对正常小鼠肝组织进行免疫组织化学分析。（图 E）使用免疫组化试剂盒 M&R HRP/DAB Detection IHC Kit，抗体1:100 稀释；(图F)使用另一种试剂盒，抗体 1:100 稀释。
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