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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
Hexokinase II Antibody
- 抗原:
synthetic peptide corresponding to the sequence of human hexokinase (isoform II)
- 应用范围:
W
- 宿主:
Rabbit
- 适应物种:
H,Mk
- 级别:
详见MSDS文件
- 保质期:
详见说明书
- 库存:
大量
- 供应商:
CST
- 是否单克隆:
2
- 保存条件:
-20°c
- 规格:
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格: | 产品价格: | ¥请询价 | |
|---|---|---|---|
| 规格: | 100 ul (10 western blots) | 产品价格: | ¥请询价 |
| 规格: | carrier free & custom formulation / quantity | 产品价格: | ¥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H Mk | Endogenous | 102 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Hexokinase II Antibody detects endogenous levels of total hexokinase (isoform II) protein. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human hexokinase (isoform II). Antibodies are purified by protein A and peptide affinity chromatography. |
| Background | Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III, and IV (glucokinase), have been identified. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Protein A Purification of Antibody
1. Reagents(1) Affi-gel Protein-A Agarose (BioRad #153-6153)(2) MAPS II Binding Buffer (BioRad # 153-6161)(3) 0.314 g/ml diH2O(4) MAPS II Elution Buffer (BioRad #153-6162)(5) 0.023 g/ml diH2O(6) MAPS II Regeneration Buffer (BioRad #153-6166(7) 1M
Identification of MHC Class II Binding Peptides: Microarray and Soluble MHC Class II Molecules
antibody (mAb). Positive events (MHC class II/peptide complexes) are normalized and available for pattern analysis.
Immunohistochemical Detection of Angiotensin II Receptor
for cell-specific localization of receptor expression within tissue. The resolution and sensitivity achieved with IHC is far greater than that obtained with classic autoradiography techniques although antireceptor antibody
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