- 询价
- Cell Signaling Technology
- 2719
- USA
- 2025年10月21日
- W, IP, IF-IC
- Rabbit
- H,Mk
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- 详细信息
- 技术资料
- 抗体英文名：
βIG-H3 Antibody
- 抗原：
synthetic peptide corresponding to residues near the carboxyl terminus of human βIG-H3
- 应用范围：
W, IP, IF-IC
- 宿主：
Rabbit
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 适应物种：
H,Mk
- 库存：
大量
- 供应商：
CST
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IF-IC | H (Mk) | Endogenous | 70 | Rabbit |
| Protocols | |
|---|---|
| Specificity / Sensitivity | βIG-H3 Antibody detects endogenous levels of total βIG-H3 protein. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxyl terminus of human βIG-H3. Antibodies were purified by peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from A549 and OVCAR8 cells, untreated or treated with TGF-β overnight, using βIG-H3 Antibody. IF-IC
Confocal immunofluorescent analysis of A549 cells, untreated (left) or TGF-β-treated (right), using βIG-H3 Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). |
| Background | βIG-H3 (TGFBI/RGD-CAP/Kerato-epithelin) is a 683-amino acid secretory protein induced by TGF-β that plays a role in cell adhesion, differentiation, and apoptosis (1-4). βIG-H3 contains an internal cysteine-rich EMI domain followed by four fasciclin-1 domains and a carboxy terminal RGD domain (1,2). It contributes to cell adhesion through interactions with integrins as well as a number of extracellular matrix (ECM) proteins including collagen, fibronectin, and laminin (5-7). ECM βIG-H3 is found in a wide variety of tissues (8-12). Mutations in the βIG-H3 gene as well as elevated protein levels are most notably associated with corneal dystrophies (13).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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