Phospho-Rb (Ser807/811) Antibo

9308:

IHC / Paraffin , Immunoprecipitation , Western Blotting

Phospho-Rb (Ser807/811) Antibody detects endogenous levels of Rb when phosphorylated at serine 807/811. The antibody may cross-react with Rb phosphorylated at Ser608.

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser807/811 of human Rb. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from human fibroblasts synchronized by serum deprivation, using Phospho-Rb (Ser807/811) Antibody. Cells were synchronized for 24 hours then released by addition of serum and harvested at the times indicated. Cell cycle progression was verified by cyclin analysis and FACS. (Provided by John Boylan, Dupont/Merck, Delaware.)

The retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).

1. Sherr, C.J. (1996) Science 274, 1672-7.
2. Nevins, J.R. (1992) Science 258, 424-9.
3. Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779-90.
4. Hu, Q.J. et al. (1990) EMBO J 9, 1147-55.
5. Knudsen, E.S. and Wang, J.Y. (1997) Mol Cell Biol 17, 5771-83.
6. Lundberg, A.S. and Weinberg, R.A. (1998) Mol Cell Biol 18, 753-61.
7. Connell-Crowley, L. et al. (1997) Mol Biol Cell 8, 287-301.
8. Kitagawa, M. et al. (1996) EMBO J 15, 7060-9.
9. Geng, Y. et al. (2001) Proc Natl Acad Sci USA 98, 194-9.

• Faenza, I. et al. (2000) A role for nuclear phospholipase c beta 1 in cell cycle control. J. Biol. Chem. 275, 30520-30524. Applications: Western Blotting
• Ren, S. and Rollins, B.J. (2004) Cyclin C/cdk3 promotes Rb-dependent G0 exit. Cell 117, 239-251. Applications: Western Blotting
• Joerges, C. et al. (2003) Induction of a caffeine-sensitive S-phase cell cycle checkpoint by psoralen plus ultraviolet A radiation. Oncogene 22, 6119-6128. Applications: Western Blotting
• Dajee, M. et al. (2003) NF-kappaB blockade and oncogenic Ras trigger invasive human epidermal neoplasia. Nature 421, 639-643. Applications: Western Blotting
• Feng, L. X. et al. (2000) Stem cell factor/c-kit up-regulates cyclin D3 and promotes cell cycle progression via the phosphoinositide 3-kinase/p70S6 kinase pathway in Spermatogonia. J. Biol. Chem. 275, 25572-25576. Applications: Western Blotting
• Pan, W. et al. (2001) A cyclin D1/cyclin-dependent kinase 4 binding site within the C domain of the retinoblastoma protein. Cancer Res. 61, 2885-2891. Applications: Western Blotting
• Lam, E. W. et al. (2000) Cyclin D3 compensates for loss of cyclin D2 in mouse B-lymphocytes activated via the antigen receptor and CD40. J. Biol. Chem. 275, 3479-3484. Applications: Western Blotting
• Zheng, Y.S. et al. (2011) Oncogene , . Applications: Western Blotting
• Abrahám, E. et al. (2011) J Exp Bot 62, 2155-68. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

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• 9917 Cell Cycle/Checkpoint Antibody Sampler Kit
• 9969 Rb Antibody Sampler Kit
• 9306 PhosphoPlus® Rb (Ser780, Ser807/811) Antibody Kit
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.