- 询价
- Cell Signaling Technology
- 7652
- USA
- 2025年10月09日
- W
- Rabbit
- H,M,R,Mk,B,Dg
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-IRAK4 (Thr345/Ser346) Antibody
- 抗原：
synthetic peptide corresponding to residues surrounding Thr345/Ser346 of human IRAK4 protein
- 应用范围：
W
- 宿主：
Rabbit
- 供应商：
CST
- 级别：
详见MSDS文件
- 库存：
大量
- 适应物种：
H,M,R,Mk,B,Dg
- 保质期：
详见说明书
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey B=Bovine Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H (M) (R) (Mk) (B) (Dg) | Endogenous | 55 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-IRAK4 (Thr345/Ser346) Antibody recognizes endogenous levels of IRAK4 protein only when phosphorylated at Thr345 and Ser346. The antibody may also react with single phosphorylation of IRAK4 at Ser346. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr345/Ser346 of human IRAK4 protein. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of serum-starved KARPAS-299 cells, untreated or treated with Human Interleukin-1β (hIL-1β) #8900 (50 ng/ml, 15 min), using Phospho-IRAK4 (Thr345/Ser346) Antibody (upper) or total IRAK4 Antibody #4363 (lower). |
| Background | Interleukin-1 (IL-1) receptor-associated kinase (IRAK) is a serine/threonine-specific kinase that can be coprecipitated in an IL-1-inducible manner with the IL-1 receptor (1). The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK3/IRAK-M, and IRAK4). The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1RI, AcP, MyD88, and IRAKs (2). IRAK undergoes autophosphorylation shortly after IL-1 stimulation. The subsequent events involve IRAK dissociation from the IL-1RI complex, its ubiquitination, and its association with two membrane-bound proteins: TAB2 and TRAF6. The resulting IRAK-TRAF6-TAB2 complex is then released into the cytoplasm where it activates protein kinase cascades, including TAK1, IKKs, and the stress-activated kinases (3). Upon IL-1R/Toll-Like Receptor ligation, IRAK1 and IRAK4 are rapidly recruited to the receptor by the adaptor MyD88 (4). IRAK1 is phosphorylated by IRAK4 at Thr209 and Thr387 (5), followed by sequential autohyperphosphorylation in various domains. |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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- 作者
- 内容
- 询问日期
文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads
neutralization of phospho-specific antibodies with phospho-peptides immobilized on magnetic beads. This technique allows for sequestration of antibody–peptide complex from the incubation solution, minimizing the risk of formation of unblocked antibodies capable
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