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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
PDHK1 (C47H1) Rabbit mAb
- 抗原:
synthetic peptide corresponding to the sequence of human PDHK1 protein
- 应用范围:
W, IP
- 库存:
大量
- 级别:
详见MSDS文件
- 保质期:
详见说明书
- 适应物种:
H,M,R,Mk
- 供应商:
CST
- 是否单克隆:
1
- 保存条件:
-20°c
- 规格:
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格: | 产品价格: | ¥请询价 | |
|---|---|---|---|
| 规格: | 100 ul (10 western blots) | 产品价格: | ¥请询价 |
| 规格: | carrier free & custom formulation / quantity | 产品价格: | ¥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 47 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | PDHK1 (C47H1) Rabbit mAb detects endogenous levels of total PDHK1 protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PDHK1 protein. |
| Background | Pyruvate generated from glycolysis is converted to acetyl-CoA by pyruvate dehydrogenase (PDH) under normoxia (1-3). This is a critical link between glycolysis and the TCA cycle (3). PDH activity is regulated by phosphorylation and dephosphorylation (3). Pyruvate dehydrogenase kinase (PDHK) phosphorylates PDH and inactivates it, whereas dephosphorylation of PDH is carried out by pyruvate dehydrogenase phosphatase to generate the active form (3). Hypoxia can directly induce pyruvate dehydrogenase kinase 1 (PDHK1) expression, which results in inactivation of PDH and the TCA cycle and subsequent suppression of metabolism (4). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验组织化学分析。(图 C)使用Anti-Erk1/2 Mouse mAb 鼠单抗进行目的蛋白检测;(图 D) 使用p44/42 MAPK (Erk1/2)Rabbit mAb 兔单抗进行目的蛋白检测。 图 7 免疫组化实验检测Akt表达 注:使用 Anti-Akt (pan) Rabbit mAb 对正常小鼠肝组织进行免疫组织化学分析。(图 E)使用免疫组化试剂盒 M&R HRP/DAB Detection IHC Kit,抗体1:100 稀释;(图F)使用另一种试剂盒,抗体 1:100 稀释。
h)即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复:简单易行效果好,CST 推荐使用微波炉完成修复。合适的修复液:根据抗体说明书使用合适的修复液。用柠檬酸修复后,切片需浸泡在修复液中,自然冷却;而用 EDTA 修复后,切片可直接从修复缸中取出,直接进行下一步。注:使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb(#4267),EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
到 PVDF 膜上,同时减少蛋白不必要降解,这对于最终获得清晰、可信结果也是需要考虑因素。抗体信息:1.ACC1, Recombinant Rabbit monoclonal IgG. HuaAn. HuaAn biotechnology , inc.2.ATGL, Mouse mAb IgG1, Cat#:RT1058. HuaAn biotechnology , inc.3.p-PERK(Thr981), Rabbit Polyclonal IgG primary antibodies, Cat
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