- ￥2299 - 5599
- Sino Biological
- 北京
- SEK50251
- 2025年08月12日
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- 文献和实验
- 技术资料
- 保存条件：
在-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Mouse PCSK9 ELISA Pair Set
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
5 Plates/15 Plates
| 规格： | 5 Plates | 产品价格： | ￥2299.0 |
|---|---|---|---|
| 规格： | 15 Plates | 产品价格： | ￥5599.0 |
反应种属：Mouse
试剂对靶点：PCSK9
试剂对应用：ELISA
试剂对保存条件：Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围：54.69-3500
试剂对标记类型：HRP
包被抗体：0.2 mg/mL of rabbit anti-Mouse PCSK9 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 50251-R214)
检测抗体：0.2 mg/mL of rabbit anti-Mouse PCSK9 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.4 μg/mL in detection antibody dilution buffer before use.
试剂灵敏度：The minimum detectable dose of Mouse PCSK9 was determined to be approximately 62.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理：The Mouse PCSK9 ELISA Pair Set is for the quantitative determination of Mouse PCSK9. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for PCSK9 coated on a 96-well plate. Standards and samples are added to the wells, and any PCSK9 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-PCSK9 polyclonal antibody is then added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of PCSK9 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
试剂对检测限：54.69 pg/ml
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文献和实验1, Sun L, et al. Activation of Adiponectin Receptor Regulates Proprotein Convertase Subtilisin/Kexin Type 9 Expression and Inhibits Lesions in ApoE-Deficient Mice.Arteriosclerosis, thrombosis, and vascular biology, PubMed ID: 28546220
2, Wang D, et al. Ascorbic acid enhances low-density lipoprotein receptor expression by suppressing proprotein convertase subtilisin/kexin 9 expression.The Journal of biological chemistry, PubMed ID: 32913121
3, Yong Li, et al. Methionine Metabolism Dictates PCSK9 Expression and Antitumor Potency of PD-1 Blockade in MSS Colorectal Cancer. Advanced science (Weinheim, Baden-Wurttemberg, Germany), PubMed ID: 40125618
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