Human CD208 / DC-LAMP / LAMP3 ELISA Pair Set(产品说明)
反应种属:Human
试剂对靶点:LAMP3
试剂对应用:ELISA
试剂对保存条件:Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围:31.25-2000
试剂对标记类型:HRP
包被抗体:1 mg/mL of rabbit anti-LAMP3 monoclonal antibody, Dilute to a working concentration of 2.0 μg/mL in CBS before coating. (Catalog: # 10527-R020)
检测抗体:0.5 mg/mL rabbit anti-LAMP3 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.1 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10527-R002)
试剂灵敏度:The minimum detectable dose of Human CD208 / DC-LAMP / LAMP3 was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理:The Human CD208 / DC-LAMP / LAMP3 ELISA Pair Set is for the quantitative determination of Human CD208 / DC-LAMP / LAMP3. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CD208 / DC-LAMP / LAMP3 coated on a 96-well plate. Standards and samples are added to the wells, and any CD208 / DC-LAMP / LAMP3 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-CD208 / DC-LAMP / LAMP3 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of CD208 / DC-LAMP / LAMP3 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
试剂对检测限:31.25 pg/ml
北京义翘神州科技股份有限公司(Sino Biological Inc.)是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司,主要业务包括重组蛋白、抗体、基因和培养基等产品,以及重组蛋白、抗体的开发和生物分析检测等服务。