- ￥2299 - 5599
- Sino Biological
- 31.25 pg/ml
- 北京
- SEK10518
- 2025年08月12日
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- 详细信息
- 技术资料
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 检测范围：
31.25 pg/ml
- 检测方法：
ELISA
- 应用：
ELISA
- 适应物种：
Human
- 标记物：
HRP
- 样本：
见说明书
- 规格：
5 Plates/15 Plates
| 规格： | 5 Plates | 产品价格： | ￥2299.0 |
|---|---|---|---|
| 规格： | 15 Plates | 产品价格： | ￥5599.0 |
Human IL3RA / CD123 ELISA Pair Set（产品说明）
反应种属：Human
试剂对靶点：IL3Ra
试剂对应用：ELISA
试剂对保存条件：Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围：31.25-2000
试剂对标记类型：HRP
包被抗体：0.35 mg/mL of mouse anti-IL3RA monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 10518-MM01)
检测抗体：0.5 mg/mL rabbit anti-IL3RA monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10518-R012)
试剂灵敏度：The minimum detectable dose of Human IL3RA / CD123 was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理： The human IL3RA / CD123 ELISA Pair Set is for the quantitative determinationof human IL3RA / CD123. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for IL3RA / CD123 coated on a 96-well plate. Standards and samples are added to the wells,and any IL3RA / CD123 present binds to the immobilized antibody. The wells arewashed and a horseradish peroxidase conjugated rabbit anti-IL3RA / CD123 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of IL3RA / CD123 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of themicrowell are read at 450 nm.
试剂对检测限：31.25 pg/ml
反应种属：Human
试剂对靶点：IL3Ra
试剂对应用：ELISA
试剂对保存条件：Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围：31.25-2000
试剂对标记类型：HRP
包被抗体：0.35 mg/mL of mouse anti-IL3RA monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 10518-MM01)
检测抗体：0.5 mg/mL rabbit anti-IL3RA monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10518-R012)
试剂灵敏度：The minimum detectable dose of Human IL3RA / CD123 was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理： The human IL3RA / CD123 ELISA Pair Set is for the quantitative determinationof human IL3RA / CD123. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for IL3RA / CD123 coated on a 96-well plate. Standards and samples are added to the wells,and any IL3RA / CD123 present binds to the immobilized antibody. The wells arewashed and a horseradish peroxidase conjugated rabbit anti-IL3RA / CD123 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of IL3RA / CD123 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of themicrowell are read at 450 nm.
试剂对检测限：31.25 pg/ml
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Human IL3RA / CD123 ELISA Pair Set
￥2299 - 5599
