- ￥2299 - 5599
- Sino Biological
- 7.81 pg/ml
- 北京
- SEK10221
- 2025年08月12日
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- 详细信息
- 技术资料
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 检测范围：
7.81 pg/ml
- 检测方法：
ELISA
- 应用：
ELISA
- 适应物种：
Human
- 标记物：
HRP
- 样本：
见说明书
- 规格：
5 Plates/15 Plates
| 规格： | 5 Plates | 产品价格： | ￥2299.0 |
|---|---|---|---|
| 规格： | 15 Plates | 产品价格： | ￥5599.0 |
Human Leptin / LEP ELISA Pair Set（产品说明）
反应种属：Human
试剂对靶点：LEP
试剂对应用：ELISA
试剂对保存条件：Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围：7.81-500
试剂对标记类型：HRP
包被抗体：0.5 mg/mL of mouse anti-Human Leptin / LEP monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10221-MM03)
检测抗体：0.2 mg/mL of mouse anti-Human Leptin / LEP monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 1 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10221-MM05T)
试剂灵敏度：The minimum detectable dose of Human Leptin / LEP was determined to be approximately 7.81 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理：The Human Leptin / LEP ELISA Pair Set is for the quantitative determination of Human Leptin / LEP. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human Leptin / LEP coated on a 96-well plate. Standards and samples are added to the wells, and any Human Leptin / LEP present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-Human Leptin / LEP monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human Leptin / LEP present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
试剂对检测限：7.81 pg/ml
反应种属：Human
试剂对靶点：LEP
试剂对应用：ELISA
试剂对保存条件：Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围：7.81-500
试剂对标记类型：HRP
包被抗体：0.5 mg/mL of mouse anti-Human Leptin / LEP monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10221-MM03)
检测抗体：0.2 mg/mL of mouse anti-Human Leptin / LEP monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 1 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10221-MM05T)
试剂灵敏度：The minimum detectable dose of Human Leptin / LEP was determined to be approximately 7.81 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理：The Human Leptin / LEP ELISA Pair Set is for the quantitative determination of Human Leptin / LEP. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human Leptin / LEP coated on a 96-well plate. Standards and samples are added to the wells, and any Human Leptin / LEP present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-Human Leptin / LEP monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human Leptin / LEP present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
试剂对检测限：7.81 pg/ml
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Human Leptin / LEP ELISA Pair Set
￥2299 - 5599
