- ￥1560
- Sino Biological
- 北京
- 51019-M08HL
- 2025年08月12日
- WB
- HEK293 Cells
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A DNA sequence encoding the mouse DPP7 (Q9ET221) (Met1-Arg 506) was expressed, with a C-terminal polyhistidine tag.
- 亚型：
见说明书
- 形态：
液体
- 保存条件：
4℃
- 克隆性：
无
- 标记物：
见说明书
- 适应物种：
Mouse
- 保质期：
12个月
- 抗原来源：
见说明书
- 目录编号：
51019-M08HL
- 级别：
免疫学
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 宿主：
HEK293 Cells
- 应用范围：
WB
- 浓度：
见说明书
- 靶点：
DPP7
- 抗体英文名：
Mouse DPP7 / DPPII / DPP2 HEK293 Cell Lysate (WB positive control)
- 抗体名：
Mouse DPP7 / DPPII / DPP2 HEK293 Cell Lysate (WB positive control)
- 规格：
300 µg
反应种属：Mouse
裂解液靶点：DPP7
裂解液应用：WB
裂解液保存条件：Store at 4℃. After re-dissolution, aliquot and store at -80℃.
裂解液产品描述：Human Cell lysate that Mouse DPP7 / DPPII transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
裂解液表达宿主：HEK293 Cells
裂解液制备方法：Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解液Buffer：Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
裂解液质控信息：12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
裂解液使用建议：1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
裂解液稳定性：Samples are stable for up to twelve months from date of receipt.
裂解液使用说明：Western blot (WB): Use at an assay dependent dilution. Other Applications: Not tested. Optimal dilutions/concentrations should be determined by the end user.
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文献和实验：表达产物 蛋白名称（可保密）： 蛋白分子量：29 KDa WB用膜类型、孔径：PVDF 转膜方式（恒压、恒流）：0.3 A 恒流 转膜时间：80 min 转膜设备: 湿转 Bio-Rad 蛋白来源：cell lysate, culture medium 蛋白名称（可保密）： 蛋白分子量：15-50 kD WB用膜类型、孔径：NC，PVDF， 0.45 um 转膜方式（恒压、恒流）：恒压， semi
. Incubate tubes on ice for 15 min. 3. Clear cell lysate by centrifuging samples at 18,000× g in arefrigerated tabletop microcentrifuge for 15 min at 4°C. Transfer supernatant to a fresh microcentrifuge tube. 4. Measure proteins concentration
Background Information of RNAi
Background Information of RNA i RNA interference (RNA i) is a biological process in which the introduction of double-stranded RNA (dsRNA ) into a cell results in targeted post-tranional gene silencing. Historically, RNA i has been used as a tool
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