- ￥1560
- Sino Biological
- 北京
- 40280-V08HL
- 2025年08月12日
- WB
- HEK293 Cells
- HCV
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A DNA sequence encoding the Hepatitis C virus (serotype 1a, isolate H77) E2 protein (NP_751921.1) (Glu1-Glu278) was expressed with a polyhistidine tag at the C-terminus.
- 亚型：
见说明书
- 形态：
液体
- 保存条件：
4℃
- 克隆性：
无
- 标记物：
见说明书
- 适应物种：
HCV
- 保质期：
12个月
- 抗原来源：
见说明书
- 目录编号：
40280-V08HL
- 级别：
免疫学
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 宿主：
HEK293 Cells
- 应用范围：
WB
- 浓度：
见说明书
- 靶点：
HCV-E2
- 抗体英文名：
Hepatitis C virus (HCV-1a) E2 HEK293 Cell Lysate (WB positive control)
- 抗体名：
Hepatitis C virus (HCV-1a) E2 HEK293 Cell Lysate (WB positive control)
- 规格：
300 µg
反应种属：HCV
裂解液靶点：HCV-E2
裂解液应用：WB
裂解液保存条件：Store at 4℃. After re-dissolution, aliquot and store at -80℃.
裂解液产品描述：Human Cell lysate that Hepatitis C virus (HCV-1a) E2 transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
裂解液表达宿主：HEK293 Cells
裂解液制备方法：Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解液Buffer：Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
裂解液质控信息：12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
裂解液使用建议：1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
裂解液稳定性：Samples are stable for up to twelve months from date of receipt.
裂解液使用说明：Western blot (WB): Use at an assay dependent dilution. Other Applications: Not tested. Optimal dilutions/concentrations should be determined by the end user.
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文献和实验Construction and Characterization of Adenovirus Vectors
C. Cells: A549 Cells: 293, low-passage Grow 293 and A549 cells in 150-mm dishes in a 37°C, 5% CO2 incubator and split 1:2 or 1:3 when they reach ~90% confluence. Cells: 293N3S Maintain 293N3S cells in 150-mm dishes
Studies of the Ubiquitin Proteasome System
from Qiagen; Talon from Clontech; or equivalent product from Invitrogen) 50 mM sodium phosphate, pH 8.0 ( appendix 2A ) Bacterial lysate containing E2 protein ( protocol 37
〔5〕。HCV属于单股正连RNA病毒，其基因组包膜糖蛋白区E2/NS1基因组C端相对保守，N端含有两个高变区域(Hypervariable region，HVR)即HVR1(384～410/413 aa)和HVR2(474～480 aa)。研究发现HVR1含有病毒株特异的中和性抗原表位，它的变异与丙肝慢性化及防治失败密切相关〔6〕。HCV感染者体内可同时存在大量基因序列相关的HCV准种株群体，而且会随病情和/或病程不同而发生变化〔7，8〕。这种序列异质性变异株可发生在HCV基因组的各区段，包括5′-非编
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