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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A DNA sequence encoding the extracellular domain of human RSV Fusion glycoprotein (P03420.1) (Met 1-Thr 529) was expressed, fused with a polyhistidine tag at the C-terminus.
- 亚型:
见说明书
- 形态:
液体
- 保存条件:
4℃
- 克隆性:
无
- 标记物:
见说明书
- 适应物种:
RSV
- 保质期:
12个月
- 抗原来源:
见说明书
- 目录编号:
11049-V08BL
- 级别:
免疫学
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 宿主:
Baculovirus-Insect cells
- 应用范围:
WB
- 浓度:
见说明书
- 靶点:
RSV-F
- 抗体英文名:
Human respiratory syncytial virus (RSV) (A2) Fusion glycoprotein / RSV-F Insect Cell Lysate (WB positive control)
- 抗体名:
Human respiratory syncytial virus (RSV) (A2) Fusion glycoprotein / RSV-F Insect Cell Lysate (WB positive control)
- 规格:
300 µg
反应种属:RSV
裂解液靶点:RSV-F
裂解液应用:WB
裂解液保存条件:Store at 4℃. After re-dissolution, aliquot and store at -80℃.
裂解液产品描述:Baculovirus-Insect Cell lysate that Human RSV (A2) Fusion glycoprotein / RSV-F transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
裂解液表达宿主:Baculovirus-Insect cells
裂解液制备方法:Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解液Buffer:Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
裂解液质控信息:12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
裂解液使用建议:1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
裂解液稳定性:Samples are stable for up to twelve months from date of receipt.
裂解液使用说明:Western blot (WB): Use at an assay dependent dilution. Other Applications: Not tested. Optimal dilutions/concentrations should be determined by the end user.
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文献和实验Respiratory Syncytial Virus and Other Pediatric Respiratory Virus Infections
but a relatively small number are responsible for the majority of significant illness. Of these the most commonly diagnosed in the pediatric population is respiratory syncytial virus (RSV), which infects essentially all children in their first or second year
Sequences derived from the respiratory syncytial virus (RSV) fusion (F) protein were expressed in insect cells as recombinant glutathione-S -transferase (GST)-tagged proteins. The sequence covering the F2 subunit (GST-F2), and a truncated
Rapid and accurate detection of respiratory syncytial virus (RSV) provides pathogen-specific diagnosis, allows implementation of appropriate infection control measures, and improves patient management. One diagnostic challenge
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