- ￥1560
- Sino Biological
- 北京
- 10982-H02HL
- 2025年08月12日
- WB
- HEK293 Cells
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A DNA sequence encoding the human CD2 (NP_001758.2) (Met1-Asp209) was expressed with the Fc region of human IgG1 at the C-terminus.
- 亚型：
见说明书
- 形态：
液体
- 保存条件：
4℃
- 克隆性：
无
- 标记物：
见说明书
- 适应物种：
Human
- 保质期：
12个月
- 抗原来源：
见说明书
- 目录编号：
10982-H02HL
- 级别：
免疫学
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 宿主：
HEK293 Cells
- 应用范围：
WB
- 浓度：
见说明书
- 靶点：
CD2
- 抗体英文名：
Human CD2 CHO Cell Lysate (WB positive control)
- 抗体名：
Human CD2 CHO Cell Lysate (WB positive control)
- 规格：
300 µg
反应种属：Human
裂解液靶点：CD2
裂解液应用：WB
裂解液保存条件：Store at 4℃. After re-dissolution, aliquot and store at -80℃.
裂解液产品描述：HEK293 Cell Human CD2 transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
裂解液表达宿主：HEK293 Cells
裂解液制备方法：Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解液Buffer：Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
裂解液质控信息：12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
裂解液使用建议：1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
裂解液稳定性：Samples are stable for up to twelve months from date of receipt.
裂解液使用说明：Western blot (WB): Use at an assay dependent dilution. Other Applications: Not tested.
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文献和实验Generation of Dendritic Cells from Lymphoid Precursors
-1, the transcription factors Pax5 and Ikaros, and represent the earliest recognizable human B-cell precursor identified in human BM (7 ,8 ). BM cells of CD34+ Lin- CD10+ phenotype can differentiate into antigen-presenting DC under the appropriate culture conditions
线粒体荧光探针大全：TMRM，Mitotracker，JC-1
are the cause of several human diseases, indicating the importance of overall morphology for cell functioning (see Note 12.2 "Technical Focus: Mitochondria in Diseases"). Organelle morphology is also controlled by cytoskeletal elements, including actin filaments
Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
.L., Golan, D.E., Zhu, D.M., Miller, J.M., Meier, W., Davies, E.A., and van der Merwe, P.A. 1997. Low affinity interaction of human or rat T cell adhesion molecule CD2 with its ligand aligns adhering membranes to achieve high physiological affinity. J
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