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- B6179
- 2025年08月29日
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- 文献和实验
- 技术资料
- 英文名：
Bongkrekic acid solution
- CAS号：
11076-19-0 (principal component)
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文献和实验Methods for the Detection of D-Amino-Acid Oxidase
on the activity of the samples. It was terminated by the addition of 1 ml of 10% trichloroacetic acid. In a blank, trichloroacetic acid was added to the reaction mixture before the enzyme reaction was started. The precipitate was removed by centrifugation (700 x g
Methods for the Detection of D-Amino-Acid Oxidase
phosphate, and 1 mM EDTA, pH 7.7), 5 x Denhardt's solution ( 8 ), and 0.5% SDS] containing 20 μg/ml denatured, fragmented salmon sperm DNA. The D-amino-acid oxidase cDNA fragment amplified above by PCR was labeled with [-32P]dCTP (~110 TBq/mmol, Amersham
使用接种于指示细胞之步骤。 · 特点︰简单、经济与灵敏，广泛使用，可作为例行之侦测步骤。可以侦测不易培养之支原体，例如M. hyorhinis，较直接培养法快，约一星期即可知道结果。 · 缺点：有时仍会有萤光背景，影响判读。 材料︰ · Hanks’ balanced salt solution without NaHCO3 or phenol red (HBSS, GibcoBRL 21250-014﹚、bisbenzimide (Hoechst No.33258, Sigma B
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