Mouse IL1A / IL-1A / IL-1F1 ELISA Kit(产品说明)
反应种属:小鼠
试剂盒靶点:IL1A
试剂盒应用:ELISA
试剂盒保存:Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
试剂盒介绍:For the quantitative determination of Mouse IL1A / IL-1A / IL-1F1 concentration in serum and cell culture supernates.
试剂盒线性范围:6.56-420
试剂盒包被抗体类型:rabbit mAb
试剂盒检测抗体类型:rabbit pAbKIT50114
试剂盒检测灵敏度:3.11
试剂盒天然样本检测量:The average concentration of Mouse IL1A in 10 normal mouse serum is 77.89 +/- 55.74 pg/mL ranging from 17.41 to 165.15 pg/mL. Mouse splenocytes (10E6 cells/mL) were cultured for 3 days in RPMI supplemented with 5% fetal bovine serum and stimulated with 5 ug/mL LPS. Aliquots of the cell culture supernates were removed and assayed for levels of natural mouse IL1A, and measured 8.11 pg/mL.
试剂盒特异性:This assay recognizes both recombinant and natural Mouse IL1A. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
试剂盒检测原理:Solid Phase Sandwich ELISA
试剂盒组成:1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
试剂盒检测样品:血清,细胞上清液