- ￥3200
- Sino Biological
- 3.11
- 北京
- KIT50114
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 检测范围：
3.11
- 检测方法：
Solid Phase Sandwich ELISA
- 应用：
ELISA
- 适应物种：
Mouse
- 标记物：
见说明书
- 样本：
血清,细胞上清液
- 规格：
1 Kit (96 Tests)
反应种属：小鼠
试剂盒靶点：IL1A
试剂盒应用：ELISA
试剂盒保存：Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
试剂盒介绍：For the quantitative determination of Mouse IL1A / IL-1A / IL-1F1 concentration in serum and cell culture supernates.
试剂盒线性范围：6.56-420
试剂盒包被抗体类型：rabbit mAb
试剂盒检测抗体类型：rabbit pAbKIT50114
试剂盒检测灵敏度：3.11
试剂盒天然样本检测量：The average concentration of Mouse IL1A in 10 normal mouse serum is 77.89 +/- 55.74 pg/mL ranging from 17.41 to 165.15 pg/mL. Mouse splenocytes (10E6 cells/mL) were cultured for 3 days in RPMI supplemented with 5% fetal bovine serum and stimulated with 5 ug/mL LPS. Aliquots of the cell culture supernates were removed and assayed for levels of natural mouse IL1A, and measured 8.11 pg/mL.
试剂盒特异性：This assay recognizes both recombinant and natural Mouse IL1A. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
试剂盒检测原理：Solid Phase Sandwich ELISA
试剂盒组成：1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
试剂盒检测样品：血清,细胞上清液
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文献和实验人转录因子E2F1 ( E2F1 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 E2F1 单抗包被于酶标板上，标准品和样品中的 E2F1与单抗结合，加入生物素化的抗人 E2F1 ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入底物工作液显蓝色，最后加终止液硫酸，在 450nm 处测 OD 值，E2F1 浓度与 OD 值成正比
A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
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