Mouse GRO Beta / CXCL2 ELISA Kit(产品说明)
反应种属:小鼠
试剂盒靶点:CXCL2
试剂盒应用:ELISA
试剂盒保存:Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
试剂盒介绍:For the quantitative determination of Mouse GRO Beta / CXCL2 concentration in serum and cell culture supernates.
试剂盒线性范围:4.69-300
试剂盒包被抗体类型:rabbit mAb
试剂盒检测抗体类型:rabbit pAbKIT50070
试剂盒检测灵敏度:2.35
试剂盒天然样本检测量:EL-4 mouse lymphoblast cells (1 x 10E6 cells/mL) were cultured in DMEM supplemented with 10% fetal calf serum, , stimulated with 10 μg/mL PHA and 10 ng/mL PMA for 2-3 days.Aliquots of the cell culture supernates were removed and assayed for levels of natural CXCL2, no detectable levels ware observed.The average concentration of Mouse CXCL2 in 10 normal mouse serum is 72.18 +/- 46.53 pg/mL ranging from 28.21 to 156.59 pg/mL.
试剂盒特异性:This assay recognizes both recombinant and natural Mouse CXCL2. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
试剂盒检测原理:Solid Phase Sandwich ELISA
试剂盒组成:1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
试剂盒检测样品:血清,细胞上清液
1, Che, D; et al. p-Phenylenediamine induces immediate contact allergy and non-histaminergic itch via MRGPRX2. Chemico-biological interactions, Pubmed: 34826398
2, Che, D; et al. Action of substance P and PAMP(9-20) on different excitation sites of MRGPRX2 induces differences in mast cell activation. International immunopharmacology, Pubmed: 34753104