- ￥3200
- Sino Biological
- 3.19-250
- 北京
- KIT12047
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 检测范围：
3.19-250
- 应用：
ELISA
- 适应物种：
人
- 样本：
细胞上清液
- 规格：
1 Kit (96 Tests)
反应种属：人
试剂盒靶点：IL17A
试剂盒应用：ELISA
试剂盒保存：Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
试剂盒介绍：For the quantitative determination of Human IL17A/IL-17A/IL17 concentration in serum and cell culture supernates.
试剂盒线性范围：1.88-120
试剂盒包被抗体类型：mouse mAb
试剂盒检测抗体类型：mouse mAbKIT12047
试剂盒检测灵敏度：0.58
试剂盒天然样本检测量：Human peripheral blood mononuclear cells （1 x 10E6 cells/mL） were cultured in RPMI supplemented with 10% fetal calf serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate, stimulated with 10 μg/mL PHA for 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of natural IL17A, and measured 22.86 pg/mL.
试剂盒特异性：This assay recognizes both recombinant and natural Human IL17A. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.Cross-reactivity was observed to be 2.3% with 50 ng/mL of recombinant Rat IL17F.
试剂盒检测原理：Solid Phase Sandwich ELISA
试剂盒组成：1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
试剂盒检测样品：细胞上清液
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文献和实验2, Wu, C; et al. Influence of Th17/Treg balance on progression of liver cirrhosis in patients with chronic hepatitis B. International Journal of Clinical and Experimental Medicine, Pubmed: 22745730
目前自动洗板机应用比较广泛，凡是具有酶联免疫吸附技术（ELISA）的酶反应板均可使用。应用范围有：肿瘤标记物的检测、传染性疾病的检查、人类免疫缺陷病毒（HIV）抗体的检测、沙眼衣原体感染的血清学检查和TORCH感染的血清学检查等。应用ELISA完成这些方面的检测后，使用先进的全自动洗板机进行洗板，以保证测试的准确性和提高实验的可靠性。 自动洗板机是进行酶联免疫实验时，检测各种感染性病毒所必备的设备之一，其作用是通过清洗，实现酶联免疫实验过程中结合相与游离相的分离。当前，酶标洗板
ELISPOT (Enzyme-linked ImmunoSPOT)
-specific responses, applicable to a wide range of areas of immunology research, including cancer, transplantation, infectious disease, and vaccine development. Human IL-17
Scanning the human genome with combinatorial transcription factor libraries Published online: 18 February 2003, doi:10.1038/nbt794 March 2003 Volume 21 Number 3 pp 269 - 274 Pilar Blancafort, Laurent Magnenat & Carlos F
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