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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
3个月
- 英文名:
Plasmodium falciparum (strain 3D7) CSP Gene ORF cDNA clone expression plasmid, C-GFPSpark tag
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1 Unit
| 货号: | 表达载体: |
| VG40295-ACG | pCMV3-C-GFPSpark |
| VG40295-CH | pCMV3-C-His |
| VG40295-CM | pCMV3-C-Myc |
| VG40295-CY | pCMV3-C-HA |
| VG40295-NF | pCMV3-SP-N-FLAG |
| VG40295-NH | pCMV3-SP-N-His |
| VG40295-NM | pCMV3-SP-N-Myc |
| VG40295-NY | pCMV3-SP-N-HA |
| VG40295-UT | pCMV3-untagged |
| VG40295-ACR | pCMV3-C-OFPSpark |
Plasmodium falciparum (strain 3D7) CSP Gene ORF cDNA clone expression plasmid, C-GFPSpark tag产品信息
基因靶点:CSP
反应种属:P. falciparum
应用说明:Stable or Transient mammalian expression
cDNA描述:Full length Clone DNA of Plasmodium falciparum (strain 3D7) circumsporozoite protein with C terminal GFPSpark tag.
| 货号: | VG40295-ACG |
| 产品名称: | Plasmodium falciparum (strain 3D7) CSP Gene ORF cDNA clone expression plasmid, C-GFPSpark tag |
| NCBI 参考序列号: | XM_001351086.1 |
| 序列长度: | 1194 |
| 表达载体: | pCMV3-C-GFPSpark |
| cDNA描述: | Full length Clone DNA of Plasmodium falciparum (strain 3D7) circumsporozoite protein with C terminal GFPSpark tag. |
| 货号: | VG40295-CH |
| 产品名称: | Plasmodium falciparum (strain 3D7) CSP Gene ORF cDNA clone expression plasmid, C-His tag |
| NCBI 参考序列号: | XM_001351086.1 |
| 序列长度: | 1239 |
| 表达载体: | pCMV3-C-His |
| cDNA描述: | Full length Clone DNA of Plasmodium falciparum (strain 3D7) circumsporozoite protein with C terminal His tag. |
| 货号: | VG40295-CM |
| 产品名称: | Plasmodium falciparum (strain 3D7) CSP Gene ORF cDNA clone expression plasmid, C-Myc tag |
| NCBI 参考序列号: | XM_001351086.1 |
| 序列长度: | 1239 |
| 表达载体: | pCMV3-C-Myc |
| cDNA描述: | Full length Clone DNA of Plasmodium falciparum (strain 3D7) circumsporozoite protein with C terminal Myc tag. |
| 货号: | VG40295-CY |
| 产品名称: | Plasmodium falciparum (strain 3D7) CSP Gene ORF cDNA clone expression plasmid, C-HA tag |
| NCBI 参考序列号: | XM_001351086.1 |
| 序列长度: | 1194 |
| 表达载体: | pCMV3-C-HA |
| cDNA描述: | Full length Clone DNA of Plasmodium falciparum (strain 3D7) circumsporozoite protein with C terminal HA tag. |
| 货号: | VG40295-NF |
| 产品名称: | Plasmodium falciparum (strain 3D7) CSP Gene ORF cDNA clone expression plasmid, N-Flag tag |
| NCBI 参考序列号: | XM_001351086.1 |
| 序列长度: | 1194 |
| 表达载体: | pCMV3-SP-N-FLAG |
| cDNA描述: | Full length Clone DNA of Plasmodium falciparum (strain 3D7) circumsporozoite protein with N terminal Flag tag. |
| 货号: | VG40295-NH |
| 产品名称: | Plasmodium falciparum (strain 3D7) CSP Gene ORF cDNA clone expression plasmid, N-His tag |
| NCBI 参考序列号: | XM_001351086.1 |
| 序列长度: | 1233 |
| 表达载体: | pCMV3-SP-N-His |
| cDNA描述: | Full length Clone DNA of Plasmodium falciparum (strain 3D7) circumsporozoite protein with N terminal His tag. |
| 货号: | VG40295-NM |
| 产品名称: | Plasmodium falciparum (strain 3D7) CSP Gene ORF cDNA clone expression plasmid, N-Myc tag |
| NCBI 参考序列号: | XM_001351086.1 |
| 序列长度: | 1194 |
| 表达载体: | pCMV3-SP-N-Myc |
| cDNA描述: | Full length Clone DNA of Plasmodium falciparum (strain 3D7) circumsporozoite protein with N terminal Myc tag. |
| 货号: | VG40295-NY |
| 产品名称: | Plasmodium falciparum (strain 3D7) CSP Gene ORF cDNA clone expression plasmid, N-HA tag |
| NCBI 参考序列号: | XM_001351086.1 |
| 序列长度: | 1194 |
| 表达载体: | pCMV3-SP-N-HA |
| cDNA描述: | Full length Clone DNA of Plasmodium falciparum (strain 3D7) circumsporozoite protein with N terminal HA tag. |
| 货号: | VG40295-UT |
| 产品名称: | Plasmodium falciparum (strain 3D7) CSP natural Gene ORF cDNA clone expression plasmid |
| NCBI 参考序列号: | XM_001351086.1 |
| 序列长度: | 1194 |
| 表达载体: | pCMV3-untagged |
| cDNA描述: | Full length Clone DNA of Plasmodium falciparum (strain 3D7) circumsporozoite protein. |
| 货号: | VG40295-ACR |
| 产品名称: | Plasmodium falciparum (strain 3D7) CSP Gene ORF cDNA clone expression plasmid, C-OFPSpark / RFP tag |
| NCBI 参考序列号: | XM_001351086.1 |
| 序列长度: | 1194 |
| 表达载体: | pCMV3-C-OFPSpark |
| cDNA描述: | Full length Clone DNA of Plasmodium falciparum (strain 3D7) circumsporozoite protein with C terminal OFPSpark / RFP tag. |
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文献和实验的克隆方法将目的基因克隆进入门载体。 (2)混合包含目的基因的入门克隆和合适的目的载体以及Gateway LR Clonase酶,构建表达克隆。(表达克隆用来在合适的宿主中进行蛋白的表达和分析。) 有几种方法可以构建Gateway入门克隆。无论您选择何种方法,创建的入门克隆都是准备用来与各种目的载体进行重组。 (1)PCR克隆(定向TOPO克隆至入门载体或与供载体B×P重组) (2)限制性内切酶消化和连接进入入门载体 (3)使用pCMV•SPORT6或pEXP-AD502*构建
Strategies to Optimize Protein Expression in E. coli
, H., Dahlgren, L.G., Flores, A., Hammarstroem, M., Johansson, I., Kotenyova, T., Nilsson, M., Nordlund, P., and Weigelt, J. 2008b. The use of systematic N‐ and C‐terminal deletions to promote production and structural studies of recombinant proteins
and genomic information 96. Full-Malaria http://fullmal.ims.u-tokyo.ac.jp/ Full-length cDNA library from erythrocytic-stage Plasmodium falciparum 97. GeneCards http://bioinfo.weizmann.ac.il/cards/ Integrated database of human genes,maps, proteins
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