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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
3个月
- 英文名:
Human ITIH6 Gene ORF cDNA clone expression plasmid
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1 Unit
基因靶点:ITIH6
反应种属:Human
应用说明:Stable or Transient mammalian expression
cDNA描述:Full length Clone DNA of Human inter-alpha-trypsin inhibitor heavy chain family, member 6
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文献和实验Anatomy of a Comparative Gene Expression Study
such as EST's. While neither of these methods will produce DNA's for every human gene, both can yield enough different expressed sequences to make substantial arrays. Both types of DNA have been used before in array-like applications: cDNA libraries were used
Anatomy of a Comparative Gene Expression Study
, and the kinds of biological questions which they can help to answer. The illustration below shows the steps that make up a comparative cDNA hybridization experiment. Click on any of the steps in the image to jump to an explanation
通过2类方法 :一是感染性病毒颗粒感染宿主细胞,二是通过脂质体法、显微注射法 、磷酸钙共沉淀法及DEAE一葡聚糖法等非病毒载体的方式将基因导入到细胞中。外源基因的体外表达一般采用质粒表达载体,如将重组质粒导入CHO细胞可建立高效稳定的表达系统,而利用COS细胞可建立瞬时表达系统。目前,病毒载体已成为动物体内表达外源基因的有力工具,在临床基因治疗的探索中也发挥了重要作用。痘苗病毒由于其基因的分子量相当大(约187kb),利用它作为载体可同时插入几种外源基因,从 而构 建多价疫苗。另外,逆转
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