The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1:500 - 1:2000
IHC
1:50 - 1:200
IF
1:50 - 1:100
Western blot analysis of extracts of mouse liver, using ID3 antibody.
Immunohistochemistry of paraffin-embedded rat brain using ID3 antibody.
Immunohistochemistry of paraffin-embedded mouse kidney using ID3 antibody.
Immunohistochemistry of paraffin-embedded mouse pancreas using ID3 antibody.
Immunohistochemistry of paraffin-embedded mouse brain using ID3 antibody.
Immunohistochemistry of paraffin-embedded mouse spleen using ID3 antibody.
Immunohistochemistry of paraffin-embedded human lung cancer using ID3 antibody.
Immunofluorescence analysis of HeLa cells using ID3 antibody.
Background:
The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with other HLH proteins. However, the encoded protein lacks a basic DNA-binding domain and therefore inhibits the DNA binding of any HLH protein with which it interacts.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.