The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1:500 - 1:2000
IHC
1:50 - 1:200
IF
1:20 - 1:50
Western blot analysis of extracts of various cell lines, using VAPB antibody.
Immunohistochemistry of paraffin-embedded human liver cancer using VAPB Antibody.
Immunohistochemistry of paraffin-embedded human liver cancer using VAPB Antibody.
Immunohistochemistry of paraffin-embedded human kidney cancer using VAPB Antibody.
Immunohistochemistry of paraffin-embedded mouse brain using VAPB Antibody.
Immunohistochemistry of paraffin-embedded mouse spleen using VAPB Antibody.
Immunofluorescence analysis of HeLa cells using VAPB antibody.
Background:
The protein encoded by this gene is a type IV membrane protein found in plasma and intracellular vesicle membranes. The encoded protein is found as a homodimer and as a heterodimer with VAPA. This protein also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.