The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1:500 - 1:2000
IHC
1:50 - 1:200
IP
1:20 - 1:50
Western blot analysis of extracts of various cell lines, using TCEB2 antibody.
Immunohistochemistry of paraffin-embedded human colon using TCEB2 antibody.
Immunohistochemistry of paraffin-embedded human esophagus using TCEB2 antibody.
Immunohistochemistry of paraffin-embedded human stomach using TCEB2 antibody.
Immunoprecipitation analysis of 150ug extracts of MCF7 cells using 3ug TCEB2 antibody.
Background:
This gene encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. Pseudogenes have been identified on chromosomes 11 and 13.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.