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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Hax1a.
- 亚型:
mouse IgG1
- 形态:
Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.02% sodium azide. Antibody was purified by Protein-A affinity chromatography.
- 保存条件:
Store at 4°C.
- 克隆性:
单克隆
- 标记物:
见下方详细说明
- 适应物种:
H, R
- 保质期:
1-2年
- 抗原来源:
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Hax1a.
- 目录编号:
253267
- 级别:
超纯
- 库存:
大量
- 供应商:
Abbiotec
- 宿主:
Mouse
- 应用范围:
E, WB
- 浓度:
见详细说明信息
- 靶点:
Hax1a (9G6C6)
- 抗体英文名:
Hax1a (9G6C6) Antibody
- 抗体名:
Hax1a (9G6C6) Antibody
- 规格:
0.1 mg
Hax1a (9G6C6) Antibody产品详细介绍:
| 产品名称: | Hax1a (9G6C6) Antibody |
| 说明书: | 【点击查看详细说明及参考图片】 |
| 货号: | 253267 |
| 规格: | 0.1 mg |
| 产品描述: | The HS-1 associated protein X-1 (Hax1) was initially identified in a yeast two-hybrid assay on the basis of its ability to bind to the hemapoietic cell-specific protein 1 (HS-1). Hax1 possesses anti-apoptotic activity and is structurally related to Bcl-2 family members, including the presence of BH1- and BH2-like domains. It has recently been shown to interact with HIV viral protein R (Vpr), a protein required for viral pathogenesis of HIV and linked to T-cell apoptosis through activation of caspases 3 and 9. Other studies indicate that Hax1-mediated processing of HtrA2 (also known as Omi) by the mitochondrial protease PARL allows survival of lymphocytes and neurons when cytokines are limiting. At least four isoforms of Hax1 are known to exist. This antibody is expected to recognize the longest isoform (Hax1a) as well as the shortest. |
| 克隆类型: | Mouse Monoclonal Antibody |
| 亚型: | mouse IgG1 |
| 免疫原: | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Hax1a. |
| 适用物种: | H, R |
| 应用范围: | E, WB |
| 应用说明: | E: 1:500-1:1,000; WB: 1:100-1:500 |
| 别名: | Hax1a; HS-1 associated protein X-1; HCLS1 associated protein X-1; HCLSBP1; HS1BP1 |
| 形态: | Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.02% sodium azide. Antibody was purified by Protein-A affinity chromatography. |
| 保存条件: | Store at 4°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment. |
| 参考文献: |
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文献和实验和酶标抗原或抗体按不同步骤与固相载体表面吸附的抗体或抗原发生反应,再用洗涤的方法分离抗原抗体复合物和游离成分,然后加入酶的作用底物催化显色,进行定性或定量。 细胞ELISA:该方法将细胞视为固相载体。多用于检测细胞表面的抗原或受体或筛选细胞表面抗原的特异性抗体。 试剂及配制 1. 洗涤液(pH 7.4 PBS) KH2PO4 0.2g Na2 HPO4--12H2 O 2.9g NaCl 8.0g KCl
[试剂及配制](1)包被液(pH9.6碳酸盐缓冲液) Na2CO3 0.16g NaHCO3 0.29g 蒸馏水加至100ml(2)洗涤液(pH7.4PBS-Tween20) KH2PO4 0.2g Na2 HPO4-12H20 2.9g NaCl 8.0g KCl 0.2g Tween20 0.5ml 蒸馏水加
实验试剂 1. 聚苯乙烯塑料微量组织培养板:4×10孔。 2. 包被液(pH9.6):NaHCO3 29.3g、Na2 CO3 19.5g,蒸馏水加至1000ml,置4℃保存。 3. 洗涤液(0.01mol/L、pH7.4PBS):NaCl 8.0g、KH2 PO4 0.2g、Na2 HPO4 •12 H2 O 2.9g、KCl 0.2g、Tween20 0.5ml,加蒸馏水至1000ml。
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