The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1:200 - 1:2000
IHC
1:50 - 1:500
Western blot analysis of extracts of various cell lines, using NBAS antibody.
Background:
This gene encodes a protein with two leucine zipper domains, a ribosomal protein S14 signature domain and a Sec39 like domain. The protein is thought to be involved in Golgi-to-ER transport. Mutations in this gene are associated with short stature, optic nerve atrophy, and Pelger-Huet anomaly.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.