美国BD采血管 单核细胞制备管362761 BD Vacut
ainer® CPT™ Mononuclear Cell Preparation Tube - Sodium Citrate 362760/362753- ¥120 - 5500
- 美国BD
- 362761
- 美国
- 2025年09月02日
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1支/10支/20支/60支
| 规格： | 1支 | 产品价格： | ¥120.0 |
|---|---|---|---|
| 规格： | 10支 | 产品价格： | ¥950.0 |
| 规格： | 20支 | 产品价格： | ¥1800.0 |
| 规格： | 60支 | 产品价格： | ¥5500.0 |
BD Vacutainer® CPT™ is a fully-closed system for separation of mononuclear cells from whole blood, where cell separation is carried out in the primary blood collection tube. This decreases the complexity of steps for mononuclear cell separation, thereby minimizing variability from sample processing.
CPT™ is an evacuated, sterile blood collection tube containing buffered sodium citrate or sodium heparin anticoagulant, liquid density medium and an inert gel barrier. It is intended for the collection of whole blood and the subsequent separation of mononuclear blood cells for the purposes of in vitro diagnostic examination.
Whole blood is drawn directly into the CPT™ using standard phlebotomy techniques and processed in the same tube. During centrifugation, the gel forms a physical barrier between the mononuclear cells in plasma and the erythrocytes and granulocytes. The separated, concentrated suspension of mononuclear cells in plasma can then be transported in the primary blood collection tube.
1.使用BD CPT™管，应在室温（18-25ºC）。
2.用BD一次性静脉采血针将血液采进入采血管。
3.收集后，将BD CPT管应被轻轻地180度上下颠倒混匀，持续8-10次
在室温下垂直储存管，直到离心。两小时内采血的血液样本离心效果最好。
离心条件：相对离心力1500g-1800g,15-20分钟，25℃室温
4.在室温下（18-25ºC），离心管/血液样本需在1500到1800的RCF（相对离心力）下离心至少20分钟。
注：离心之前，CPT管应被轻轻地180度上下颠倒混匀，持续8-10次。另外，要检查该管是放在适当的离心机中，注意要对称放置管子，以双数例如2支，4支对称放置在离心机中。
警告：过度的离心速度（超过2000 RCF）可能导致断管和血液暴露以及其他可能的损害。对于一个给定的RCF要计算正确的离心速度，使用下面的公式：
转速设定值=(（RCF）x100000)/(（1.12）X（r）)
其中r（以厘米）是从离心管底到离心中心柱的径向距离，CPT管处在水平位置，RCF是相对离心力，在这种情况下,为1500–1800g。
5．离心后，PRP位于最上层（血浆层）的底部（见图）。不干扰细胞层的情况下，可用注射器或吸管之类的抽吸出PRP部分并用于注射。
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Azoulay S, Nevers MC, Creminon C, Heripret L, Durant J, et al. Sensitive Enzyme Immunoassay for Measuring Plasma and Intracellular Nevirapine Levels in Human Immunodeficiency Virus-Infected Patients. Antimicrob Agents Chemother 2004;48:104-109.
-
Baechler E, Battliwalla F, Karypis G, Gaffney P, et al. Interferon-inducible Gene Expression Signature in Peripheral Blood Cells of Patients with Severe Lupus. PNAS 2003;100:2610-2615.
-
Balasuriya U, Snijder E, Heidner H, Zhang J, Zevenhoven-Dobbe J, Boone J, et al. Development and Characterization of an Infectious cDNA Clone of the Virulent Bucyrus Strain of Equine Arteritis Virus. J Gen Virol 2007;88:918-924.
-
Becher F, Pruvosat A, Schlemmer D, Creminon C, Goujard C, et al. Significant Levels of Intracellular Stavudine Triphosphate are Found in HIV-Infected Zidovudine-treated Patients. AIDS 2003;17:555-561.
-
Bhattacharya S, Tyagi S, Srisuma S, DeMeo D, Shapiro S, et al. Peripheral Blood Gene Expression Profiles in COPD Subjects. J Clin Bioinformat 2011;1:1-12.
-
Colombo S, Beguin A, Telenti A, Biollaz J, Buclin T, et al. Intracellular Measurements of anti-HIV Drugs Indinavir, Amprenavir, Saquinavir, Nelfinavir, Lipinavir, Atazanavir, Efavirenz and Nevirapine in Peripheral Blood Mononuclear Cells by Liquid Chromatography Coupled to Tandem Mass Spectrometry. J Chromatograph B 2005;819:259-276.
-
Cronin AJ, Aucutt-Walter NM, Budinetz T, Bonafide CP, DiVittore, NA, et al. Low Dose Infusion Does Not Impair Natural Killer Cell Function in Healthy Volunteers. Br J Anaesth 2003;91:805-809.
-
Ehrhardt M, Mock M, Haefeli W, Mikus G, Burhenne J. Monitoring of Lopinavir and Ritonavir in Peripheral Blood Mononuclear Cells, Plasma, and Ultrafiltrate using a Selective and Highly Sensitive LC/MS/MS Assay. J Chromatograph B 2007;850:249-258.
-
Fischer M, Huber W, Kalivroussis A, Ott P, Opravil M, et al. Highly Sensitive Methods for Quantitation of Human Immunodeficiency Virus Type 1 RNA from Plasma, Cells and Tissues. J Clin Microbiol 1999;37:1260-1264.
-
Green LJ, Marker P, Ray C, Cook CA, Jaken S, et al. Development and Validation of a Drug Activity Biomarker that Shows Target Inhibition in Cancer Patients Receiving Enzastaurin, a Novel Protein Kinase C-B Inhibitor. Clin Cancer Res 2006;12:3408-3415.
-
Holodniy M, Mole L, Yen-Lieberman B, Margolis D, Starkey C, et al. Comparative stabilities of Quantitative Human Immunodeficiency Virus RNA in Plasma from Samples Collected in Vacutainer CPT, Vacutainer PPT and Standard Vacutainer Tubes. J Clin Microbiol 1995;33:1562-1566.
-
Hughes A, Mattison J, Western L, Powderly J, et al. Microtube Device for Selectin-Mediated Capture of Viable Circulating Tumor Cells from Blood. Clin Chem 2012;58:846-853.
-
Katial RK, Brandt BL, Moran EE, Marks S, Agnello V, Zollinger W. Immunogenicity and Safety Testing of a Group B Intranasal Meningococcal Native Outer Membrane Vesicle Vaccine. Infect Immun 2002;70:702-707.
-
Mahony J, Chong S, Coombes B, Smieja M, Petrich A. Analytical Sensitivity, Reproducibility of Results, and Clinical Performance of Five PCR Assays for Detecting Chlamydia Pneumoniae DNA in Peripheral Blood Mononuclear Cells. J Clin Microbiol 2000;38:2622-2627
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