Coumarin 6香豆素 6,38215-36-0

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务。

Coumarin 6

CAS No. : 38215-36-0

MCE 国际站：Coumarin 6

产品活性：Coumarin 6 是一种荧光染料，可作为荧光探针用于微粒给药系统中，对活性分子给药系统进行体内跟踪、细胞摄取和转运机制研究 (λ_exc=450 nm，λ_em=505 nm)。

研究领域：Others

作用靶点：Fluorescent Dye

In Vitro: Coumarin 6 labeled HPMC nanoparticles
(1) Dissolve Coumarin 6 in DMF to prepare a 5 mg/mL organic phase solution.
(2) Dissolve hydroxypropyl methyl cellulose (HPMC) in HBSS to prepare a 50 μg/mL aqueous solution.
(3) Add the organic solution to the aqueous solution using a MasterFlex L/S pump (volume ratio of organic solution to aqueous solution is 1:100) with an injection rate of 36 mL/min.
(4) Prepare 70 nm nanoparticles by stirring at room temperature for 5 min at a stirring speed of 9 mL/min.
(5) Add 2 μg/mL nanoparticles to the MDCKII cell culture dish and incubate in the dark for 5 min, 15 min, 30 min and 60 min.
(6) Add cold PBS buffer to stop the uptake of nanoparticles by MDCKII cells and clean with HBSS.
(7) Use a fluorescence microscope to observe the uptake of nanoparticles by MDCKII cells.

相关产品：MitoSOX Red  |  BODIPY 493/503  |  Nile Red  |  D-Luciferin potassium  |  JC-1  |  FITC  |  Propidium Iodide  |  CFDA-SE  |  FITC-Dextran (MW 4000)  |  Dihydroethidium  |  DiR  |  Calcein-AM  |  Thiazolyl Blue  |  2-NBDG  |  DiI  |  Fluo-4 AM  |  TMRE  |  Cy5.5  |  Indocyanine green  |  Acridine Orange hydrochloride  |  CY5  |  BODIPY 581/591 C11  |  FM4-64  |  CY5-SE  |  PKH 26  |  DiD perchlorate  |  Diphenylterazine

热门产品线：重组蛋白  |  化合物库  |  天然产物  |  荧光染料  |  PROTAC  |  同位素标记物

Trending products：Recombinant Proteins  |  Bioactive Screening Libraries  |  Natural Products  |  Dye Reagents  |  PROTAC  |  Isotope-Labeled Compounds