Rat/Mouse hybridoma cell line
that produces antibodies that recognize MOPC 167 idiotype. 28-6-20 sec 细胞- 询价
- ATCC
- CRL-2490
- 2025年12月05日
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文献和实验The production of monoclonal antibody by hybridoma
cell culture plates. Reagents: 1-3 x 10 8-9 immune spleen cells 1-6 x10 7-8 myeloma cells in log phase of growth Complete Media No Sera (CMNS) for washing of the myeloma and spleen cells. Hybridoma medium CM - HAT
determines how much antibodies one can obtain. For example, a rabbit can provide 5 mls of blood every two weeks, a mouse provides significantly less, while a horse can provide quite a bit more. An antibodiy isolated from a single B lymphocyte cell
Hybridomas are derived from the fusion of spleen and myeloma cells and produce monoclonal antibodies. Each hybridoma cell line produces an antibody with a unique specificity allowing the production of highly defined reagents that can be used
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