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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
默瑞(上海)生物科技有限公司
- 规格:
5 U / 20 rxn sample
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文献和实验Sequencing of BAC DNA--BAC DNA测序
16 µl BigDye Terminator Ready Reaction Mix 1 µg BAC DNA (determined from gel) 10 pmol primer water to 40 µl Heat tubes at 95℃ for 5 min., then perform 30 cycles of: 95℃ x 30 sec 55℃ x 10 sec 60℃ x 4 min Run BACs on a 377 and load the entire
Thermo Sequenase I DNA polymerase (10 U/ul) 1.0 µL Distilled Water (to a final volume of 27 µL) ____ µL
: G, A, T, C). Prewarm at 37℃ in an incubator. To each annealed DNA sample (Step #2), add the following: 3.0 µL 1 x Labeling mix (dilute 5 x stock with water) 1.0 µL [35 S]dATP (1000 Ci / mmol). Allow to stand at room temperature until used
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