The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1:200 - 1:2000
IHC
1:50 - 1:200
Western blot analysis of extracts of various cell lines, using PPP1CC antibody.
Immunohistochemistry of paraffin-embedded human gastric cancer using PPP1CC antibody.
Immunohistochemistry of paraffin-embedded rat brain using PPP1CC antibody.
Immunohistochemistry of paraffin-embedded human stomach using PPP1CC antibody.
Background:
The protein encoded by this gene belongs to the protein phosphatase family, PP1 subfamily. PP1 is an ubiquitous serine/threonine phosphatase that regulates many cellular processes, including cell division. It is expressed in mammalian cells as three closely related isoforms, alpha, beta/delta and gamma, which have distinct localization patterns. This gene encodes the gamma isozyme. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.