荧光标记，乙酰化赖氨酸抗体（Acetyl Lysine An

Species: Rabbit

Formulation: 250 µg/mL antibody stored in PBS, pH 7, with 50% glycerol

Storage & Stability: Store product at –20^_℃. Three years from date of shipping.

Immunogen: Acetylated KLH conjugates

Conjugation: FITC/Ab ratio 10:1, followed by G25 filtration.

Purification: The antibody was purified on acetyl-lysine agarose.

Specificity: Recognize proteins acetylated on lysine residues.

Tested: acetylated histone, acetylated BSA, and acetylated MBP, no reaction to the non-acetylated proteins.

Application: For direct immunofluorescence experiments

Scientific Background: Acetylation of lysine is an important reversible modification. The activities of some proteins are controlled by acetylation of lysine. Histone acetyltransferases (HATs) acetylate the conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) that contain lysines residues. Histone deacetylases (HDACs) removes the acetyl group from the same residue. [1] Acetylation/deacetylation of histones results in cell signaling processes that include gene activity, cell growth, differentiation and apoptosis. [2] In cancer and polyglutamine diseases, the regulation of protein acetylation/deacetylation is impaired (7). Numerous anti-cancer drugs target HDACs. [3]

References: 1. Hassig, C.A. et al. Curr. Opin. Chem. Biol. 1997. 1, 3, 300-308. 2. Allfrey, V. G. et al. Proc. Natl. Acad. Sci. USA 1964, 51, 786-794. 3. Vigushin, D.M. et al. Curr. Cancer Drug Targets 2004, 4, 205-218.